请使用支持JavaScript的浏览器! 【求助】Jackson二抗冻干粉如何配置?-Jackson ImmunoResearch代理咨询-蚂蚁淘商城
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PhysicalState:Freeze-driedpowder
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide
SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:100-1:800
FlowCytometry:-1:100-1:800
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.
indicatedvolumeofd.water是多少水?实际使用的时候加什么稀释液配成1:100或者1:800?
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