Prospec重组人二肽基肽酶4|Human DPP4 现货
CATALOGUE NUMBER

ENZ-375

SYNONYMS
CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.
INTRODUCTION
DPP4 also called adenosine deaminase complexing protein-2, and T-cell activation antigen CD26 is a serine exopeptidase and complex enzyme that is expressed on the surface of most cell types. DPPIV is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPP4 plays a role in t-cell activation. DPP4 is associated with intracellular signal transduction, apoptosis and involved in tumor BIOLOGy. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. FurThermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).
DESCRIPTION
DPPIV Human Recombinant produced in Insect cells is a single, glycosylated polypeptide chain containing 737 amino acids (39-766) and having a molecular mass of 85.4kDa.
DPPIV is fused to a 6 His Tag at C-terminus and purified using conventional chromatography techniques.
SOURCE
Insect cells.
PHYSICAL APPEARANCE
Sterile Filtered colorless solution.
FORMULATION
DPP4 is formulated in 20mM Tris-HCl buffer pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol.
STABILITY
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
PURITY
Greater than 95.0% as determined by Analysis by SDS-PAGE.
UNIT DEFINITION
One unit will hydrolyze 1 umole of p-nitroaniline per minute at pH8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.
BIOLOGICAL ACTIVITY
>200 Units/mg.
ASSAY CONDITIONS
- Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA- Total reaction volume: 100 ul- Reaction temperature: 37°C. 1. Add the reaction buffer to each well.2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.3. Add the enzyme (DPP-4) diluent to each well.4. Incubate the 96 well plate at 37°C.5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm.20ng DPP4= 0.075 OD at 405nm.40ng DPP4= 0.125 OD at 405nm.60ng DPP4= 0.2 OD at 405nm.80ng DPP4= 0.275 OD at 405nm.
100ng DPP4= 0.35 OD at 405nm.
AMINO ACID SEQUENCE
ADPSRKTYTL TDYLKNTYRL KLYSLRWISD HEYLYKQENN ILVFNAEYGN SSVFLENSTF DEFGHSINDY SISPDGQFIL LEYNYVKQWR HSYTASYDIY DLNKRQLITE ERIPNNTQWV TWSPVGHKLA YVWNNDIYVK IEPNLPSYRI TWTGKEDIIY NGITDWVYEE EVFSAYSALW WSPNGTFLAY AQFNDTEVPL IEYSFYSDES LQYPKTVRVP YPKAGAVNPT VKFFVVNTDS LSSVTNATSI QITAPASMLI GDHYLCDVTW ATQERISLQW LRRIQNYSVM DICDYDESSG RWNCLVARQH IEMSTTGWVG RFRPSEPHFT LDGNSFYKII SNEEGYRHIC YFQIDKKDCT FITKGTWEVI GIEALTSDYL YYISNEYKGM PGGRNLYKIQ LSDYTKVTCL SCELNPERCQ YYSVSFSKEA KYYQLRCSGP GLPLYTLHSS VNDKGLRVLE DNSALDKMLQ NVQMPSKKLD FIILNETKFW YQMILPPHFD KSKKYPLLLD VYAGPCSQKA DTVFRLNWAT YLASTENIIV ASFDGRGSGY QGDKIMHAIN RRLGTFEVED QIEAARQFSK MGFVDNKRIA IWGWSYGGYV TSMVLGSGSG VFKCGIAVAP VSRWEYYDSV YTERYMGLPT PEDNLDHYRN STVMSRAENF KQVEYLLIHG TADDNVHFQQ SAQISKALVD VGVDFQAMWY TDEDHGIASS TAHQHIYTHM SHFIKQCFSL PHHHHHH.
USAGE
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.