Prospec重组人二肽基肽酶4|HumanDPP4现货
CATALOGUENUMBER

ENZ-375

SYNONYMS
CD26,ADABP,ADCP2,DPPIV,TP103,DPP4,Dipeptidylpeptidase4,DipeptidylpeptidaseIV,DPPIV,T-cellactivationantigenCD26,Adenosinedeaminasecomplexingprotein2,CD26antigen.
INTRODUCTION
DPP4alsocalledadenosinedeaminasecomplexingprotein-2,andT-cellactivationantigenCD26isaserineexopeptidaseandcomplexenzymethatisexpressedonthesurfaceofmostcelltypes.DPPIVisanintrinsicmembraneglycoproteinandaserineexopeptidasethatcleavesX-prolinedipeptidesfromtheN-terminusofpolypeptides.DPP4playsaroleint-cellactivation.DPP4isassociatedwithintracellularsignaltransduction,apoptosisandinvolvedintumorBIOLOGy.Thereareatleast63substrateswhichcanbindspecificallytoDPP4enzymeincludinggrowthfactors,chemokines,neuropeptides.FurThermore,DPP4playsamajorroleinglucosemetabolismbycleavingincretinssuchasglucose-dependentinsulinotropicpolypeptide(GIP)andglucagon-likepeptide-1(GLP-1).
DESCRIPTION
DPPIVHumanRecombinantproducedinInsectcellsisasingle,glycosylatedpolypeptidechaincontaining737aminoacids(39-766)andhavingamolecularmassof85.4kDa.
DPPIVisfusedtoa6HisTagatC-terminusandpurifiedusingconventionalchromatographytechniques.
SOURCE
Insectcells.
PHYSICALAPPEARANCE
SterileFilteredcolorlesssolution.
FORMULATION
DPP4isformulatedin20mMTris-HClbufferpH-8,100mMNaCl,1mMEDTAand10%glycerol.
STABILITY
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime. Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
PURITY
Greaterthan95.0%asdeterminedbyAnalysisbySDS-PAGE.
UNITDEFINITION
Oneunitwillhydrolyze1umoleofp-nitroanilineperminuteatpH8.0at37°Cusing1mMofGly-Prop-nitroanildeasasubstrate.
BIOLOGICALACTIVITY
>200Units/mg.
ASSAYCONDITIONS
-Reactionbuffer:20mMTrispH8.0,0.1MNaCl,1mMEDTA-Totalreactionvolume:100ul-Reactiontemperature:37°C.1.Addthereactionbuffertoeachwell.2.Addthe10ulof10mMsubstrate(Gly-Prop-nitroanilide)toeachwell.3.Addtheenzyme(DPP-4)diluenttoeachwell.4.Incubatethe96wellplateat37°C.5.Readtheopticaldensityat405nm.Opticaldensitywasmeasuredat405nmafterincubatingenzymesolutionwith1mMofp-nitroanilidasasubstrate.0ngDPP4=0ODat405nm.20ngDPP4=0.075ODat405nm.40ngDPP4=0.125ODat405nm.60ngDPP4=0.2ODat405nm.80ngDPP4=0.275ODat405nm.
100ngDPP4=0.35ODat405nm.
AMINOACIDSEQUENCE
ADPSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLPHHHHHH.
USAGE
ProSpec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.