Invitrogen 18080-044 反转录酶III SuperScript III Reverse...-蚂蚁淘商城

新闻动态

Invitrogen 18080-044 反转录酶III SuperScript III Reverse...

2024-04-28

主营产品：美国Kimble微量电动匀浆器、线粒体提取杜恩斯玻璃组织匀浆器，美国Wheaton低速磁力搅拌器、双侧壁悬浮细胞培养瓶，德国Greiner细菌接种环、培养皿、培养瓶，美国ABI荧光定量 PCR耗材注册资金：供应信息公司介绍Invitrogen 18080-044 反转录酶III SuperScript? III Reverse Transcriptase 货号： 18080-044Invitrogen? 相关应用：反转录和 cDNA 合成货号规格价格18080-044 10,000 units 4,245.0018080-0854 x 10,000 units 15,350.0018080-0932,000 units 1,220.00SuperScript? III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript? II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript? II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript? III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.? Thermostability – Half life of 220 minutes at 50°C? Yield – Reduced RNase H activity for more full-length cDNA? Specificity – Full activity at 50°C for increased specificity with Gene Specific Primers? Applications – Array labeling, cDNA libraries, RT-PCR, primer extension, and 3′ and 5′ RACESource: Purified from E. coli expressing the pol gene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.Unit Definition: One unit of SuperScript? III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as a template?primer (3).Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 μl for 10 min. at 37°C.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Optimal Reaction Temperature: 50° CFinal Product Size(s): 12.3 kb or lessReverse Transcriptase: SuperScript? IIIRibonuclease H Activity: ReducedForm: FrozenFormat: Tube(s)Quantity: 10,000 unitsTemplate: ssRNASensitivity: MediumProduct Size: 10,000 unitsConcentration: 200 U?μlEnzyme Function: RNA dependent DNA PolymeraseShipping Condition: Dry IceNumber of Reactions: 50 Reactions内容及储存SuperScript? III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.Store at -20°C. Guaranteed stable for 6 months when properly stored.**品如下：货号 ** 品名规格 15596-026 Invitrogen Trizol Reagent 100mL 15596-018 Invitrogen Trizol Reagent 200mL10296-010 Invitrogen Trizol LS Reagent 100mL10503-027 Invitrogen DNAzol Reagent 100mL10966-018 Invitrogen Platinum Taq DNA Polymerase 100U10966-034 Invitrogen Platinum Taq DNA Polymerase 500U11304-011 Invitrogen Platinum Taq High Fidelity 100 rxns11708-039 Invitrogen Platinum Pfx DNA Polymerase 500U11733-038 Invitrogen Platinum SYBR Green qPCR SuperMix-UDG 100 rxns18080-051 Invitrogen SuperScript III First-Strand System 50 rxns 11904-018 Invitrogen SuperScript II First-Strand System 50 rxns 10928-042 Invitrogen SuperScript II One-Step RT-PCR System 100 rxns 18080-044 Invitrogen SuperScript III RNase H- Reverse Transcriptase 10000U 18064-014 Invitrogen Superscript II RNase H- Reverse Transcriptase 10000U 28025-013 Invitrogen M-MLV Reverse Transcriptase 40000U 10812-014 Invitrogen Second-strand Buffer 0.5mL 10777-019 Invitrogen RNaseOUT Recombinant Ribonclease Inhibitor 5000U 48190-011 Invitrogen Random Primers 9 A260U 18427-013 Invitrogen 10 mM dNTP Mix 100 μL 18418-012 Invitrogen Oligo（dT）12-18 Primer 25 μg 15224-017 Invitrogen T4 DNA Ligase, 1 unit/μL 100U 15224-041 Invitrogen T4 DNA Ligase, 5 unit/μL 250U 11668-019 Invitrogen LipofectAMINE 2000 Reagent 1.5mL11668-027 Invitrogen LipofectAMINE 2000 Reagent 0.75mL18324-012 Invitrogen LipofectAMINE Reagent 1mL11514-015 Invitrogen PLUS Reagent 0.85mL18292-011 Invitrogen Lipofectin 1mL12252-011 Invitrogen OligofectAMINE Reagent 1mL31985-062 Invitrogen Opti-MEM I Reduced Serum Medium 100mL31985-070 Invitrogen Opti-MEM I Reduced Serum Medium 500mLS866 Invitrogen Streptavidin1mL 10748-010 Invitrogen BenchMark Prestained Protein Ladder 2x250μL17504-044 B-27? Serum-Free Supplement(50X)，liquid17100-017 Collagenase,Type I,powder17101-015 Collagenase,Type II,powder17104-019 Collagenase,Type IV,powder欢迎来到北京明阳科华生物科技有限公司网站，我公司位于拥有6项遗产，拥有文化遗产项目数较多的城市，一座有着三千余年建城历史、八百六十余年建都史的历史文化名城，拥有众多历史名胜古迹和人文景观的中国\"八大古都”之一 —北京。具体地址是北京朝阳朝阳区北苑路13号，联系人是吴烦。主要经营美国Kimble微量电动匀浆器、线粒体提取杜恩斯玻璃组织匀浆器，美国Wheaton低速磁力搅拌器、双侧壁悬浮细胞培养瓶，德国Greiner细菌接种环、培养皿、培养瓶，美国ABI荧光定量 PCR耗材，法国Hyphen-biomed发色底物，美国W.S.Tyler筛分仪，加拿大Lablabs无菌采样袋，美国Millipore细胞电阻仪，美国康宁Transwell小室，英国Carbosynth核糖、核苷。单位注册资金未知。

电话：010-59919166

传真：010-59919166

手机：13701245989

地址：北京朝阳朝阳区北苑路13号

网址：http://mingyangkehua.cn.b2b168.com/

本文链接： https://www.ebiomall.com/b478-wheaton/info-1441227672.html

免责声明本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。

没有了

wheaton杜恩斯Dounce型组织研磨器 357538 357544 授权代理

2014-03-28

　Wheaton W348923-F 348252 台式MINI型贴壁细胞培养滚瓶机

2015-04-24