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来源于美国，英国，法国，德国，澳洲，以色列等地区
- 药准字：
- 主要组成成分：mobitec的巨大芽孢杆菌表达系统
- 性状：mobitec的巨大芽孢杆菌表达系统
- 适应症：mobitec的巨大芽孢杆菌表达系统
- 规格：mobitec的巨大芽孢杆菌表达系统
- 贮藏：mobitec的巨大芽孢杆菌表达系统
- 有效期：36
- 生产企业：德国
hp-vector表达系统megaterium芽孢杆菌
hp-vector expression systems for bacillusmegaterium
features:
high performance vectors with optimized sequence
protein yield up to 10 times enhanced compared toprotein expression with basic plasmid
plasmids with established mcs
encoding c- or n-terminal his-tag for versatilepurification (native, 6xhis-tag)
secretion with lipa or yoch signal peptide up to 9fold increased
optimized high performance (hp) vectors for bacillusmegaterium!
mobitec provides a wide range of useful vectors forthe b. megaterium system that are adaptable to most applicationsand protein purification methods. these include vectors carrying asecretion signal peptide sequence and a 6xhis-tag.
with our new hp-vectors yields up to 10-fold highercompared to the basic plasmids are possible. all plasmids haveestablished multiple cloning sites (mcs) for versatile cloning.furthermore we offer vectors encoding c- or n-terminal his-tags foreasy purification. the protein secretion with lipa or yoch signalpeptides is up to nine-fold increased. induction of proteinexpression of all vectors is achieved by the tightly regulated andefficiently inducible xylose operon.
versatile system with a wide range of vectors
the b. megaterium expression system provides aversatile and easy-to-handle tool for stable and high-yield proteinproduction, both small- and large-scale.
b. megaterium has proven to be an excellentalternative host to e. coli for heterologous gene expression.unlike other bacilli strains, proteolytic degradation by alkalineproteases is avoided. in addition, there are no endotoxins found inthe cell wall.
b. megaterium protoplasts specifically optimized fortransformation
the protoplasts supplied by mobitec are from b.megaterium strain wh320, developed by prof. dr. w. hillen at theinstitute of microbiology in erlangen, germany. these protoplastsare prepared according to an optimized protocol resulting in thehighest transformation efficiencies.
for secretion vectors we offer protoplasts of strainms941. both strains are mutants of dsm319, where wh320 is achemically mutant, while ms941 has a defined deletion in the geneof the major extracellular protease nprm.
order information, shipping and storage
please click on the respective order # for furtherdetails.
order# description amount
bmeg02 bacillus megaterium protoplasts ready fortransformation (strain wh320;1)
material is sufficient for 4 transformations pluscontrol experiment. 5 x 500 µl
bmeg50 bacillus megaterium protoplasts, strain ms9415 x 500 µl
bmeg31 pc-his1623hp shuttle vector, c-term.6xhis-tag; lyophilized dna 10 μg
bmeg32 pn-his-tev1623hp shuttle vector, n-term.his-tag,tev-site 10 μg
bmeg30 p3stop1623hp, shuttle vector, lyophilized dna10 μg
bmeg33 psplipa-hp, shuttle vector with signalsequence, lypophilized dna 10 μg
bmeg34 pspyoch-hp, shuttle vector with signalsequence, lypophilized dna 10 µg
shipped at rt, protoplasts shipped on dry ice
store lyophilized vectors at 4°c, reconstitutedvectors at -20°c, protoplasts at -70°c
all vectors are e. coli / b. megaterium shuttlevectors
please note that protoplasts of bacillus megateriumhave a limited shelf life of some months only, depending on thedate of production. therefore purchase and use of protoplasts mustbe thoroughly planned.
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