请使用支持JavaScript的浏览器! 求加精:美国MoBiTec GmbH公司 MoBiTec公司,哪里有卖?-MoBiTec品牌咨询
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  • question 求加精:美国MoBiTec GmbH公司 MoBiTec公司,哪里有卖?

    蚂蚁淘生物作为MoBiTecX品牌 优质供货商,为地区客户提供优质服务!
    MoBiTec GmbHX品牌上海地区,Mo Bi TecX品牌北京地区,X品牌广东地区,质优价廉,到货快!一站式采购专家-蚂蚁淘生物
    X一级代理,X品牌总代理;正品保证,价格优惠!
    来源于美国,英国,法国,德国,澳洲,以色列等地区


    • 药准字:
    • 主要组成成分:mobitec的巨大芽孢杆菌表达系统
    • 性状:mobitec的巨大芽孢杆菌表达系统
    • 适应症:mobitec的巨大芽孢杆菌表达系统
    • 规格:mobitec的巨大芽孢杆菌表达系统
    • 贮藏:mobitec的巨大芽孢杆菌表达系统
    • 有效期:36
    • 生产企业:德国

    hp-vector表达系统megaterium芽孢杆菌
    hp-vector expression systems for bacillusmegaterium
    features:
    high performance vectors with optimized sequence
    protein yield up to 10 times enhanced compared toprotein expression with basic plasmid
    plasmids with established mcs
    encoding c- or n-terminal his-tag for versatilepurification (native, 6xhis-tag)
    secretion with lipa or yoch signal peptide up to 9fold increased
    optimized high performance (hp) vectors for bacillusmegaterium!
    mobitec provides a wide range of useful vectors forthe b. megaterium system that are adaptable to most applicationsand protein purification methods. these include vectors carrying asecretion signal peptide sequence and a 6xhis-tag.
    with our new hp-vectors yields up to 10-fold highercompared to the basic plasmids are possible. all plasmids haveestablished multiple cloning sites (mcs) for versatile cloning.furthermore we offer vectors encoding c- or n-terminal his-tags foreasy purification. the protein secretion with lipa or yoch signalpeptides is up to nine-fold increased. induction of proteinexpression of all vectors is achieved by the tightly regulated andefficiently inducible xylose operon.
    versatile system with a wide range of vectors
    the b. megaterium expression system provides aversatile and easy-to-handle tool for stable and high-yield proteinproduction, both small- and large-scale.
    b. megaterium has proven to be an excellentalternative host to e. coli for heterologous gene expression.unlike other bacilli strains, proteolytic degradation by alkalineproteases is avoided. in addition, there are no endotoxins found inthe cell wall.
    b. megaterium protoplasts specifically optimized fortransformation
    the protoplasts supplied by mobitec are from b.megaterium strain wh320, developed by prof. dr. w. hillen at theinstitute of microbiology in erlangen, germany. these protoplastsare prepared according to an optimized protocol resulting in thehighest transformation efficiencies.
    for secretion vectors we offer protoplasts of strainms941. both strains are mutants of dsm319, where wh320 is achemically mutant, while ms941 has a defined deletion in the geneof the major extracellular protease nprm.
    order information, shipping and storage
    please click on the respective order # for furtherdetails.
    order# description amount
    bmeg02 bacillus megaterium protoplasts ready fortransformation (strain wh320;1)
    material is sufficient for 4 transformations pluscontrol experiment. 5 x 500 µl
    bmeg50 bacillus megaterium protoplasts, strain ms9415 x 500 µl
    bmeg31 pc-his1623hp shuttle vector, c-term.6xhis-tag; lyophilized dna 10 μg
    bmeg32 pn-his-tev1623hp shuttle vector, n-term.his-tag,tev-site 10 μg
    bmeg30 p3stop1623hp, shuttle vector, lyophilized dna10 μg
    bmeg33 psplipa-hp, shuttle vector with signalsequence, lypophilized dna 10 μg
    bmeg34 pspyoch-hp, shuttle vector with signalsequence, lypophilized dna 10 µg
    shipped at rt, protoplasts shipped on dry ice
    store lyophilized vectors at 4°c, reconstitutedvectors at -20°c, protoplasts at -70°c
    all vectors are e. coli / b. megaterium shuttlevectors
    please note that protoplasts of bacillus megateriumhave a limited shelf life of some months only, depending on thedate of production. therefore purchase and use of protoplasts mustbe thoroughly planned.
    蚂蚁淘 2020-11-10
    answer全部回答 (共 2 条)
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