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苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司，以常规生物学实验外包与生物学试剂为基础，放眼于生命科学领域的前沿技术服务，公司技术骨干有着多年的生物学科研背景，毕业于国内外名牌大学，并且有着丰富的项目经验，了解国内外科研行情，我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点，和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括：抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人，认真做事，服务至上“，希望用我们的专业知识和项目经验为国内科研客户解决科研问题，祝您科研之路畅通无阻！
- 药准字：
- 主要组成成分：mobitec的巨大芽孢杆菌表达系统
- 性状：mobitec的巨大芽孢杆菌表达系统
- 适应症：mobitec的巨大芽孢杆菌表达系统
- 规格：mobitec的巨大芽孢杆菌表达系统
- 贮藏：mobitec的巨大芽孢杆菌表达系统
- 有效期：36
- 生产企业：德国
hp-vector表达系统megaterium芽孢杆菌
hp-vector expression systems for bacillusmegaterium
features:
high performance vectors with optimized sequence
protein yield up to 10 times enhanced compared toprotein expression with basic plasmid
plasmids with established mcs
encoding c- or n-terminal his-tag for versatilepurification (native, 6xhis-tag)
secretion with lipa or yoch signal peptide up to 9fold increased
optimized high performance (hp) vectors for bacillusmegaterium!
mobitec provides a wide range of useful vectors forthe b. megaterium system that are adaptable to most applicationsand protein purification methods. these include vectors carrying asecretion signal peptide sequence and a 6xhis-tag.
with our new hp-vectors yields up to 10-fold highercompared to the basic plasmids are possible. all plasmids haveestablished multiple cloning sites (mcs) for versatile cloning.furthermore we offer vectors encoding c- or n-terminal his-tags foreasy purification. the protein secretion with lipa or yoch signalpeptides is up to nine-fold increased. induction of proteinexpression of all vectors is achieved by the tightly regulated andefficiently inducible xylose operon.
versatile system with a wide range of vectors
the b. megaterium expression system provides aversatile and easy-to-handle tool for stable and high-yield proteinproduction, both small- and large-scale.
b. megaterium has proven to be an excellentalternative host to e. coli for heterologous gene expression.unlike other bacilli strains, proteolytic degradation by alkalineproteases is avoided. in addition, there are no endotoxins found inthe cell wall.
b. megaterium protoplasts specifically optimized fortransformation
the protoplasts supplied by mobitec are from b.megaterium strain wh320, developed by prof. dr. w. hillen at theinstitute of microbiology in erlangen, germany. these protoplastsare prepared according to an optimized protocol resulting in thehighest transformation efficiencies.
for secretion vectors we offer protoplasts of strainms941. both strains are mutants of dsm319, where wh320 is achemically mutant, while ms941 has a defined deletion in the geneof the major extracellular protease nprm.
order information, shipping and storage
please click on the respective order # for furtherdetails.
order# description amount
bmeg02 bacillus megaterium protoplasts ready fortransformation (strain wh320;1)
material is sufficient for 4 transformations pluscontrol experiment. 5 x 500 µl
bmeg50 bacillus megaterium protoplasts, strain ms9415 x 500 µl
bmeg31 pc-his1623hp shuttle vector, c-term.6xhis-tag; lyophilized dna 10 μg
bmeg32 pn-his-tev1623hp shuttle vector, n-term.his-tag,tev-site 10 μg
bmeg30 p3stop1623hp, shuttle vector, lyophilized dna10 μg
bmeg33 psplipa-hp, shuttle vector with signalsequence, lypophilized dna 10 μg
bmeg34 pspyoch-hp, shuttle vector with signalsequence, lypophilized dna 10 µg
shipped at rt, protoplasts shipped on dry ice
store lyophilized vectors at 4°c, reconstitutedvectors at -20°c, protoplasts at -70°c
all vectors are e. coli / b. megaterium shuttlevectors
please note that protoplasts of bacillus megateriumhave a limited shelf life of some months only, depending on thedate of production. therefore purchase and use of protoplasts mustbe thoroughly planned.
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