Contributor: Suprya JayadevDate: December 13, 19941) Transfect cells.2) Culture cells 1-3 passages in a T-75 flask containing selection material (e.g. hygromycin, neomycin, etc)3) Pellet cells and resuspend at a concentration of 1 x 105 cells/ml in selection media.4) Aliquot 1 drop/well of cell suspension in to each well of a 96-well tissue culture plate.--> NOTE: Maintain 2-10 plates for each transfection.5) Check plates every day, if a population grows in a well then subculture cells by diluting 1:10 with selection media and seeding as shown: 6) Continue subculturin in this manner for 5-10 passages to insure that a clonal population is established.
行业新闻
- 苏美达国际技术贸易有限公司手术显微镜国际招标公告(1)0664-1840SUMECT23
- TwistDx公司RPA试剂盒进口
- 1st BASE公司产品介绍【代理商代购现货】
- 美国EZ Biosystems公司提供最有效的转染试剂
- Cell Projects公司产品介绍【代理商代购现货】
- 工作时间
- frontiersci公司产介绍【代理代购现货】
- usbio品牌单抗501371 Rabbit Anti-CHK1中英文实验说明
- R&D Systems公司807-UK-100/Recombinant Human uPAR Protein现货促销
- usbio品牌144325-HRP Rabbit Anti-CD44中英文说明书
- AMSBIO公司介绍|代理商整理AMS Biotechnology
- Glen Research核苷提取试剂特约代理商
Selection of Transfected Suspension Cells
