Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium’s PCR master mix delivers superior performance even with the most challenging samples tested. For detailed information, click the download tab to view the protocol and product flyer pdf files. The master mix is offered in three formats containing different amounts of ROX reference dye for use with different PCR instruments (without ROX, low ROX, and high ROX). View the product protocol to find the master mix that is recommended for your instrument.

EvaGreen dye and applications are covered under patent US patent nos. 7,803,943 and 7,776,567 and pending international patents. Cheetah Taq is covered by pending US and international patents.

References

Mao, et al. Characterization of EvaGreen Dye and the implication of its physicochemical properties for qPCR applications. BMC Biotechnology 7, 76 (2007).

Download a list of Selected EvaGreen References.