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Molecular Devices/EarlyTox Caspase-3/7 R110 Assay Kit/Explorer Kit - PN: R8346/4858337219
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产品说明
This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)2-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity. Available Configurations: ConfigurationDescriptionPart NumberExplorer Kit(1) vial of Rhodamine 110 (80 µM, 1 mL)(2) vials of caspase-3 inhibitor (5 mM, 20 µL)(2) vials of DEVD-R110 (2 mM, 500 µL)(2) vials of lysis buffer (10 mL)Each kit contains enough material for two (2) 96-well or 384-well microplates.(R8346)
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