产品说明
Description:REAADSProteinSAntigenisanenzyme-linkedimmunosorbentassay(ELISA)forthequantitativedeterminationofTotalandFreeProteinSAntigenincitratedhumanplasma.Advantages:TotalandFreeproteinSConvenientELISAprocedureReagentcompletekit– noextraexpenseforadditionalreagents–costeffectiveSixpointreferencecurveAccuratemeasurementofaspecificproteinNoreconstitutionrequired,reducingriskofdilutionerrorsKitreagentsoptimizedformoreaccurateresultsPlateandcomponentsarestableafteropeningthroughshelf-life–costeffectiveCalibratedforProteinC,SandvWFassaystoISTHstandardsandoptimizedforeachlotformoreaccurateresultsAssayformatsavailableforbothmanualandselectautomatedplatformsTotalincubationtime:60minutesKitComposition:Reagents12x8anti-humanProteinSantibodycoatedmicrowells60mlSampleDiluent(blue-greensolution);containssodiumazide.3vialsx0.5mllyophilizedReferencePlasma,withassaysheet.12mlanti-humanProteinSHRPConjugate(redsolution).13mlSubstrate(TMBandH2O2).15mlStoppingSolution(0.36Nsulfuricacid).30mlWashConcentrate(33XPBSwith0.01%Tween20).Note:turbiditymayappearinwashconcentratewhichwillnotaffectcomponentperformanceandshoulddisappearwhenworkingdilutionisprepared.2mlFreeProteinSReagent(PEG).Storeat2–8°C.DoNotFreeze.MaterialsRequiredbutnotSuppliedProteinSControlPlasma(Totaland/orFree).ReconstituteControlPlasmaselectedforusefollowingmanufacturer’sinstructions,andstoreasrecommended.Reagentgradewater(1L)topreparePBS/Tweenwashsolution,toreconstituteReferencePlasma,andtozeroorblanktheplatereaderduringthefinalassaystep.GraduatedcylindersPrecisionpipettorscapableofdeliveringbetween5and1000microliters,withappropriatetipsMiscellaneousglasswareappropriateforsmallvolumehandlingFlaskorbottle,1literWashbottles,preferablywiththetippartiallycutbacktoprovideawidestream,oranautomatedorsemi-automatedwashingsystemDisposablegloves,powder-freerecommendedPlatereADIngspectrophotometercapableofreadingabsorbanceat450nm(witha650nmreferenceifavailable)Multichannelpipettorscapableofdeliveringto8wellssimultaneouslyMicrodilutiontubesforpatientsamplepreparationCentrifugePrincipleandProcedure:PrincipleTheREAADSProteinSAntigentestkitisadoubleantibodycaptureassayformeasuringtotalandfreeProteinSlevelsinhumanplasma,expressedinrelativepercent(%)ofnormal.TheassayisintendedtobeusedasanaidinthediagnosisofProteinSdeficiencyinpatientswiththromboticdisorders.TheREAADSProteinSAntigentestkitwillaccuratelydetectantigenlevelsaslowas5%ofnormal.ProcedureDilutedcitratedpatientplasmaisincubatedinmicrowellscoatedwithcaptureantibodyspecificforhumanProteinS,allowingpatientProteinStobindtothesurface.ThehumanProteinSdetectionantibodyisadded.Afterincubation,thewellsarewashed,substrateisaddedandcolordevelopmentismeasuredinaspectrophotometerat450nmfollowingtheadditionofastopsolution.PatientProteinSlevelsaredeterminedfromasix-pointcurvepreparedfromthereferenceplasmaprovidedinthekit.FreeProteinSlevelscanbemeasuredsimultaneouslybytestingsamplesthathavebeenpretreatedwithPEG,followingthesameassayprocedure.Totalincubationtimeis60minutes.Performance:ClinicalPerformancePlasmasamplesfromhealthyblooddonorsandfrompatientswithahistoryofthrombosisweretestedtodefineandcomparetheclinicalperformanceofREAADSProteinSELISAwithawellestablished,commerciallyavailableProteinSAntigenRocketEIDmethod.Asshowninthetable,theresultscorrelatedwell,andwereshowntobestatisticallysimilarbysinglefactorANOVA.REAADSRocketEIDTotalProteinSHealthyMean105%98%Range64–150%65–143%PatientsMean59%58%Range23–140%23–148%Free ProteinSHealthyMean97%102%Range61–162%62–160%PatientsMean47%52%Range12–115%20–127%Correlation(r)=0.934;Pvalue=0.346TechnicalPerformanceIntra-assayprecisionis10.1%forREAADSTotalProteinS,and6.6%fortheFreeProteinSassay.Inter-assayprecisionis11.0%forTotal,and10.5%forFreeProteinS.Linearity,expressedasthecoefficientofdetermination(r2)is0.985forTotal,and0.992forFreeProteinS.Meanaccuracyis101%forTotal,and98%forFreeProteinSassays.REAADSProteinSELISAisarapid,convenient,highlyaccurateandprecisemethodforthequantitativedeterminationofProteinSlevelsinhumanplasma.Background:ProteinSisavitaminK-dependentproteinsynthesizedintheliver,vascularendothelium,andmegakaryocytes,whichplaysanimportantphysiologicroleintheProteinCAnticoagulantSystem.Thisanticoagulantsystemisoneofthemajorregulatorsofhemostasisbyinhibitingclotformationandbypromotingfibrinolysis.ProteinSfunctionsasacofactorforactivatedProteinConthevascularmembranetofacilitatethedegradationofclottingfactorsVaandVIIIa,down-regulatingclotformation.Innormalplasmaapproximately40%ofProteinScirculatesasafreemolecule,while60%iscomplexedwithC4b,aplasmaproteinoftheclassicalcomplementpathway.OnlyFreeProteinSisfunctionallyactiveandabletobindtoactivatedProteinC,whilethecomplexedformofProteinSisnot.ProteinSdeficiency,eithercongenitaloracquired,mayleadtoseriousthromboticeventssuchasthrombophlebitis,deepveinthrombosis,orpulmonaryembolism.TheprevalenceofProteinSdeficiencyhasbeenestimatedtobelessthan1caseper300inthegeneralpopulation.Two-thirdsofpatientswithacongenitaldeficiencyofProteinS(levelslessthan50%ofnormal)maypresentwithvenousthrombosisinyoungadulthood.Inyoungpatients(<35years)withahistoryofthrombosis,theprevalencemaybeashighas15to18%.7AcquiredProteinSdeficiencymaybeseenduringpregnancy,oralcontraceptiveororalanticoagulanttherapy,liverdisease,diabetesmellitus,postoperativecomplications,septicemiaandvariousinflammatorysyndromes.AdecreasedProteinSactivityinplasmamaybetheresultoflowconcentrationsorabnormalfunctionoftheProteinSmolecule.ThelaboratorydiagnosisofProteinSdeficiencymayrequirebothquantitativeandqualitative(functional)determinations.QuantitativedeterminationsofProteinSAntigenarebasedonimmunologicproceduressuchasradialimmunodiffusioningel,Laurellrocketimmunoelectrophoresisandenzyme-linkedimmunosorbentassay(ELISA).9,10ELISAproceduresarelesslaborintensiveandofferseveraladvantagesincludingmoreobjective,accurateandreproducIBLeresults.Inaddition,ELISAallowsautomationwithcommonlyavailablelaboratoryinstrumentation.MeasurementofplasmalevelsofbothTotalandFreeProteinSareusefultodeterminethetypeofdefectinpatientswithProteinSdeficiency.
Diapharma使命宣言
位于俄亥俄州西切斯特的Difarma Group,Inc.在诊断和研究领域销售止血、血栓形成、血小板功能测试、仪器和凋亡产品,并提供强大的技术能力和经验,以确保满足或超过客户的期望。
地黄止血显色凝块酶联免疫吸附试验试剂盒
历史
1997年1月1日,由俄亥俄州富兰克林市的Pharmacia Hepar,Inc.成立,最初是Chromogenix基质和分析的独家美国和加拿大经销商。
四分之一个多世纪前,Chromogenix开发了第一个显色底物技术,其前身是Kabi Diagnostica。卡比后来与法玛西亚合并。希帕玛目前的一些员工在法玛西亚肝素制造厂的显色部门工作。
1998年,夏帕玛搬到了俄亥俄州的西切斯特,至今仍在那里。多年来,迪法玛扩大了其产品线,包括各种止血、细胞死亡、血小板功能、生态毒理学、化验、试剂、抗体和高级制造商的仪器。
2017年,夏帕玛庆祝了20年的成功
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