Cat #: AER-051 Alternative Name EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480Lyophilized Powder Lyophilized PowderThis product is freeze dried. All water molecules have been removed.Antigen Incl. Antigen Incl.This antibody is shipped with its antigen FREE of charge!Type: PolyclonalHost: RabbitReactivity: h, m, r Immunogen - + Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.Accession (Uniprot) Number Q14246Gene ID 2015Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.Homology Mouse, Rat –11/14 amino acid residues identical. RRID AB_2756588.Purity Affinity purified on immobilized antigen.Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.Isotype Rabbit IgG.Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.Antibody concentration after reconstitution 0.8 mg/ml.Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.Negative control antigen reconstitution 100 µl double distilled water (DDW).Negative control antigen storage after reconstitution -20°C.Preadsorption Control 1 µg peptide per 1 µg antibody.Standard quality control of each lot Western blot analysis.Applications: ic, if, lci, wbMay also work in: ifc*, ih*, ip* Western blot - + Western blot analysis of rat fat (lanes 1 and 6), mouse fat (lanes 2 and 7), human Burkitt's lymphoma (Raji) (lanes 3 and 8), rat basophilic leukemia (RBL) (lanes 4 and 9) and human promyelocytic leukemia (HL-60) (lanes 5 and 10) lysates:1-5. Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (1:400).6-10. Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with the negative control antigen. Live cell imaging / Immunocytochemistry - + Expression of EMR1 in rat RBL cellsCell surface detection of EMR1 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red), (1:100). B. Live view of the cells. C. Merged image of panels A and B. References1. Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.2. McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.3. Araç, D. et al. (2012) EMBO J. 31, 1364. Scientific background - + Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.Application key:CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry,IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key: H- Human, M- Mouse, R- RatLyophilized PowderNegative Control Antigen IncludedClick here to receivea 25 µl free trial sample!Certificate of Analysis MSDSLast update: 10/11/2019Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.For research purposes only, not for human use