DescriptionCatalogueNumber05-157Replaces04-349BrandFamilyUpstateTradeNameUpstateDescriptionAnti-MAPKinase2/Erk2Antibody,clone1B3B9AlternateNamesExtracellularsignal-regulatedkinase2MAPkinase2MAPK2Mitogen-activatedproteinkinase2extracellularsignal-regulatedkinase-2mitogen-activatedproteinkinase1proteintyrosinekinaseERK2.BackgroundInformationERK2isactivatedbyMEK2,whichphosphorylatesneighboringthreonine183andtyrosine185residues.ERK2isaserine/threoninekinasethatphosphorylatesMAP2andmyelinbasicprotein.ThiskinaseisanimportantproximalcomponentoftheMAPkinasepathwayinvolvedintranseittingthesignalsfromgrowthfactors,neurotransmittersandhormonesatthecellsurfacetothetranscriptionaleventsinthenucleus.ProductInformationFormatPurifiedControlA431,HeLaandNIH/3T3celllysatesIncludedPositiveAntigenControl:Catalog#12-301,non-stimulatedA431celllysate.Add2.5µLof2-mercaptoethanol/100µLoflysateandboilfor5minutestoreducethepreparation.Load20µgofreducedlysateperlaneforminigels.PresentationPurifiedmousemonoclonalIgG2ainbuffercontainingPBSwith0.05%sodiumazide.Storefrozenat-20°C,avoidfreezethawcycles.StorageandShippingInformationStorageConditionsStablefor1yearat-20ºCfromdateofreceipt.HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.ApplicationsApplicationAnti-MAPKinase2/Erk2Antibody,clone1B3B9isanantibodyagainstMAPKinase2/Erk2foruseinIP&WB.KeyApplicationsImmunoprecipitationWesternBlottingApplicationNotesImmunoprecipitation:4μgofapreviouslotimmunoprecipitatedMAPKinase2/Erk2fromaRIPAlysatefrommouse3T3cells.Boilthecelllysateandletcoolbeforeaddingtheantibody,asthisantibodyonlybindstodenaturedMAPKinase.BIOLOGicalInformationImmunogenHPLCpurifiedmurinerecombinantMAPKinase.Clone1B3B9Clone1B3B9ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostMouseSpecificityThisantibodyrecognizesMAPkinaseencodedbythemapkgene,Mr42kDa.IsotypeIgG2aSpeciesReactivityAvianHumanMouseRatSpeciesReactivityNoteProventoreactwithHuman,Mouse,andRat.Otherspeciescrossreactivityisunknown.AntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_002745EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheMAPkinasefamily.MAPkinases,alsoknownasextracellularsignal-regulatedkinases(ERKs),actasanintegrationpointformultiplebiochemicalsignals,andareinvolvedinawidevarietyofcellularprocessessuchasproliferation,differentiation,transcriptionregulationanddevelopment.Theactivationofthiskinaserequiresitsphosphorylationbyupstreamkinases.Uponactivation,thiskinasetranslocatestothenucleusofthestimulatedcells,whereitphosphorylatesnucleartargets.Twoalternativelysplicedtranscriptvariantsencodingthesameprotein,butdifferingintheUTRs,havebeenreportedforthisgene.GeneSymbolMAPK1p38ERKP42MAPKERK2p40PRKM1p41mapkPRKM2MAPK2p42-MAPKp41ERK-2ERT1PurificationMethodProteinGChromatographyUniProtNumberP28482UniProtSummaryFUNCTION:SwissProt:P28482#Involvedinboththeinitiationandregulationofmeiosis,mitosis,andpostmitoticfunctionsindifferentiatedcellsbyphosphorylatinganumberoftranscriptionfactorssuchasELK1.PhosphorylatesEIF4EBP1;requiredforinitiationoftranslation.Phosphorylatesmicrotubule-associatedprotein2(MAP2).PhosphorylatesSPZ1(Bysimilarity).Phosphorylatesheatshockfactorprotein4(HSF4).COFACTOR:Magnesium(Bysimilarity).SIZE:360aminoacids;41390DaSUBUNIT:InteractswithMORG1(Bysimilarity).BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithHSF4.DOMAIN:SwissProt:P28482TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.PTM:DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.SIMILARITY:Belongstotheproteinkinasesuperfamily.CMGCSer/Thrproteinkinasefamily.MAPkinasesubfamily.&Contains1proteinkinasedomainMolecularWeight42kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation