DescriptionCatalogueNumber04-439BrandFamilyUpstateTradeNameUpstateDescriptionAnti-Caspase-3(Active)Antibody,rabbitmonoclonalProductInformationFormatPurifiedPresentationIn60%storagebuffer(50mMTris-Glycine(pH7.4),0.15MNaCl,0.01%sodiumazideand0.05%BSA)and40%glycerol.StorageandShippingInformationStorageConditions2yearat-20°Cfromdateofshipment.Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIlityinfreezertemperaturesbelow-20°Cmaycauseglycerol-containingsolutionstobecomefrozenduringstorage.ApplicationsApplicationPleasenotethatthisproductwillnotbeavailableforsaleafterMarch15,2015.Pleaseselectoneoftheotherantibodiesagainstthistarget.Anti-Caspase-3(Active)Antibody,RabbitdetectslevelofCaspase-3(Active)&hasbeenpublished&validatedforuseinIF,WB,IH(P).KeyApplicationsImmunofluorescenceWesternBlottingImmunohistochemistry(Paraffin)BIOLOGicalInformationImmunogenKLH-conjugatedsyntheticpeptidecomposedofresiduessubsequenttoSer29ofhumanCaspase-3.HostRabbitSpecificityRecognizesaregioncomposedofresiduessubsequenttoSer29ofhumanCaspase-3.ThisantibodywillonlydetecttheactiveformofCaspase-3IsotypeIgGSpeciesReactivityHumanMouseAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_004346.3NM_032991.2EntrezGeneSummaryThisgeneencodesaproteinwhichisamemberofthecysteine-asparticacidprotease(caspase)family.Sequentialactivationofcaspasesplaysacentralroleintheexecution-phaseofcellapoptosis.Caspasesexistasinactiveproenzymeswhichundergoproteolyticprocessingatconservedasparticresiduestoproducetwosubunits,largeandsmall,thatdimerizetoformtheactiveenzyme.Thisproteincleavesandactivatescaspases6,7and9,andtheproteinitselfisprocessedbycaspases8,9and10.Itisthepredominantcaspaseinvolvedinthecleavageofamyloid-beta4Aprecursorprotein,whichisassociatedwithneuronaldeathinAlzheimer"sdisease.Alternativesplicingofthisgeneresultsintwotranscriptvariantsthatencodethesameprotein.GeneSymbolCASP3apopainCASP-3procaspase3CPP32BSCA-1CPP-32ApopainCPP32YamaUniProtNumberP42574UniProtSummaryFUNCTION:SwissProt:P42574#InvolvedintheactivationcascadeofcaspasesresponsIBLeforapoptosisexecution.Attheonsetofapoptosisitproteolyticallycleavespoly(ADP-ribose)polymerase(PARP)ata"216-Asp--Gly-217"bond.Cleavesandactivatessterolregulatoryelementbindingproteins(SREBPs)betweenthebasichelix-loop-helixleucinezipperdomainandthemembraneattachmentdomain.Cleavesandactivatescaspase-6,-7and-9.Involvedinthecleavageofhuntingtin.SIZE:277aminoacids;31608DaSUBUNIT:Heterotetramerthatconsistsoftwoanti-parallelarrangedheterodimers,eachoneformedbya17kDa(p17)anda12kDa(p12)subunit.SUBCELLULARLOCATION:Cytoplasm.TISSUESPECIFICITY:Highlyexpressedinlung,spleen,heart,liverandkidney.Moderatelevelsinbrainandskeletalmuscle,andlowintestis.Alsofoundinmanycelllines,highestexpressionincellsoftheimmunesystem.PTM:CleavagebygranzymeB,caspase-6,caspase-8andcaspase-10generatesthetwoactivesubunits.Additionalprocessingofthepropeptidesislikelyduetotheautocatalyticactivityoftheactivatedprotease.Activeheterodimersbetweenthesmallsubunitofcaspase-7proteaseandthelargesubunitofcaspase-3alsooccurandviceversa.SIMILARITY:SwissProt:P42574##BelongstothepeptidaseC14family.MolecularWeight17kDaPhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation