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DescriptionCatalogueNumber09-849DescriptionAnti-dimethylMBP(Arg107)AntibodyAlternateNamesmyelinbasicproteinGolli-mbpMyelinmembraneencephalitogenicproteinMyelinA1protein,MBPBackgroundInformationMyelinbasicprotein(MBP)isthemajorextrinsicmembraneproteinofcentralnervoussystem.MBPisbelievedtobeimportantintheprocessofmyelinationofnervesinthecentralnervoussystem(CNS).MBPwasinitiallysequencedin1979afterisolationfrommyelinmembranes.Sincethattime,knockoutmicedeficientinMBPhavebeendevelopedwhichshoweddecreasedamountsofCNSmyelinationandaprogressivedisordercharacterizedbytremors,seizures,andearlydeath.ThegeneforMBPisonchromosome18;theproteinlocalizestotheCNSandtovariouscellsofthehematopoieticsystem.ThepoolofMBPinthecentralnervoussystemisverydiverse.NotonlyisMBPpresentinanumberofsplicevarients,buttheproteinalsoundergoesavarietyofpost-translationalmodifications,andexistsintheCNSinanumberofforms(i.e.phosphorylated,methylated,deamidatedandcitrullinated).InterestinMBPhascenteredonitsroleindemyelinatingdiseases,particularlymultiplesclerosis(MS).SeveralstudieshaveshownaroleforantibodiesagainstMBPinthepathogenesisofMS.SomestudieshavelinkedageneticpredispositiontoMStotheMBPgene,thoughamajorityhavenot.ProductInformationFormatSerumControlmouseMBPknockoutandwildtypethymuslysatesPresentationUnpurifiedrabbitpolyclonalinserumcontaining0.05%sodiumazide.StorageandShippingInformationStorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponreceiptandpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.ApplicationsApplicationAnti-dimethylMBP(Arg107)AntibodyisanantibodyagainstdimethylMBP(Arg107)foruseinWB.KeyApplicationsWesternBlottingBIOLOGicalInformationImmunogenKLH-conjugatedlinearpeptidecorrespondingtotheC-terminusofMBPdimethylatedatArg107.EpitopeMBPdimethylatedatArg107HostRabbitSpecificityThisantibodyrecognizesMBPdimethylatedatArg107.IsotypeIgGSpeciesReactivityMouseHumanRatSpeciesReactivityNoteDemonstratedtoreactwithmouse.Predictedtoreactwithhumanandratbasedon100%sequencehomology.AntibodyTypePolyclonalAntibodyEntrezGeneNumberNP_001020252EntrezGeneSummaryTheproteinencodedbytheclassicMBPgeneisamajorconstituentofthemyelinsheathofoligodendrocytesandSchwanncellsinthenervoussystem.However,MBP-relatedtranscriptsarealsopresentinthebonemarrowandtheimmunesystem.ThesemRNAsarisefromthelongMBPgene(otherwisecalled"Golli-MBP")thatcontains3additionalexonslocatedupstreamoftheclassicMBPexons.AlternativesplicingfromtheGolliandtheMBPtranscriptionstartsitesgivesriseto2setsofMBP-relatedtranscriptsandgeneproducts.TheGollimRNAscontain3exonsuniquetoGolli-MBP,splicedin-frameto1ormoreMBPexons.TheyencodehybridproteinsthathaveN-terminalGolliaasequencelinkedtoMBPaasequence.ThesecondfamilyoftranscriptscontainonlyMBPexonsandproducethewellcharacterizedmyelinbasicproteins.ThiscomplexgenestructureisconservedamongspeciessuggestingthattheMBPtranscriptionunitisanintegralpartoftheGollitranscriptionunitandthatthisarrangementisimportantforthefunctionand/orregulationofthesegenes.[providedbyRefSeq].GeneSymbolMBPmultiplesclerosisPurificationMethodUnpurifiedUniProtNumberP02686UniProtSummaryFUNCTION:TheclassicgroupofMBPisoforms(isoform4-isoform14)arewithPLPthemostabundantproteincomponentsofthemyelinmembraneintheCNS.TheyhavearoleinbothitsformationandstABIlization.Thesmallerisoformsmighthaveanimportantroleinremyelinationofdenudedaxonsinmultiplesclerosis.Thenon-classicgroupofMBPisoforms(isoform1-isoform3/Golli-MBPs)maypreferentiallyhavearoleintheearlydevelopingbrainlongbeforemyelination,maybeascomponentsoftranscriptionalcomplexes,andmayalsobeinvolvedinsignalingpathwaysinT-cellsandneuralcells.Differentialsplicingeventscombinedtooptionalpost-translationalmodificationsgiveawidespectrumofisomers,eachofthemhavingmaybeaspecializedfunction.InducesT-cellproliferation.SIZE:304aminoacids;33117DaSUBUNIT:Homodimer;isoform3existsasahomodimer.SUBCELLULARLOCATION:Myelinmembrane;Peripheralmembraneprotein;Cytoplasmicside.Note=Cytoplasmicsideofmyelin.TISSUESPECIFICITY:MBPisoformsarefoundinboththecentralandtheperipheralnervoussystem,whereasGolli-MBPisoformsareexpressedinfetalthymus,spleenandspinalcord,aswellasincelllinesderivedfromtheimmunesystem.DEVELOPMENTALSTAGE:Expressionturnsonabruptlyinfetusof14to16weeks.Evensmallerisoformsseemtobeproducedduringembryogenesis,someofthesepersistingintheadult.ExpressionofisoformMBP2ismoreevidentat16weeksanditsrelativeproportiondeclinedthereafter.PTM:SeveralchargeisomersofMBP;C1(themostcationic,leastmodified,andmostabundantform),C2,C3,C4,C5,C6,C7,C8-AandC8-B(theleastcationicform);areproducedasaresultofoptionalPTM,suchasphosphorylation,deamidationofglutamineorasparagine,argininecitrullinationandmethylation.C8-AandC8-BcontaineachtwomassisoformstermedC8-A(H),C8-A(L),C8-B(H)andC8-B(L),(H)standingforhigherand(L)forlowermolecularweight.C3,C4andC5arephosphorylated.Theratioofmethylatedarginineresiduesdecreasesinaging,makingtheproteinmorecationic.&TheN-terminalalanineisacetylated(isoform3,isoform4,isoform5andisoform6).&Arg-241wasfoundtobe6%monomethylatedand60%symmetricallydimethylated.DISEASE:Thereductioninthesurfacechargeofcitrullinatedand/ormethylatedMBPcouldresultinaweakenedattachmenttothemyelinmembrane.Thismechanismcouldbeoperativeindemyelinatingdiseasessuchaschronicalmultiplesclerosis(MS),andfulminatingMS(Marburgdisease).SIMILARITY:Belongstothemyelinbasicproteinfamily.MolecularWeightCalculatedat33kDa,themolecularweightofMBPwillvaryaccordingtospecificspliceisoformsandmodificationstates.DimethylatedMBPisobservedhereat17,20and28kDa.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation
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