DescriptionCatalogueNumberMAB2058Replaces05-283BrandFamilyChemicon®TradeNameChemiconDescriptionAnti-Integrinβ4Antibody,cloneASC-3AlternateNamesCD104ProductInformationFormatPurifiedPresentationLiquidin0.02MPBpH7.6,0.25MNaClcontaining0.1%sodiumazide.StorageandShippingInformationStorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6months.ApplicationsApplicationDetectIntegrinβ4usingthisAnti-Integrinβ4Antibody,cloneASC-3validatedforuseinFC,IP,IH.KeyApplicationsFlowCytometryImmunoprecipitationImmunohistochemistryAffectsFunctionApplicationNotesInhibitionofcellattachment:inhibitsSCC9celladhesiontohumanlamininandlamininGD-2peptide,butnottoEHSlaminin,typeIVcollagenorfibronectin.DoesnotinhibitSKOV-3celladhesiontolaminin,fibronectinortypeIVcollagen.Immunohistochemistry:onacetone-fixedfrozenhumanepithelialtissuesincludingtongue,foreskinandovary.Notforuseonformalin-fixedtissue.Flowcytometry:stainshumansquamouscellcarcinoma(SCC9),ovariancarcinoma(SKOV-3)andumbilicalveinendothelium(HUVEC).StainsRKOorK562cellstransfectedforCD104expression.Doesnotstainmonocytes,neutrophils,lymphocytes,Tcells,Bcellsorplatelets.Immunoprecipitation:precipitatesPAGEbandsof66,76,120,135and200kDa(reduced)or66,110,125,130and190kDa(non-reduced)fromsurface-biotinylatedSCC9cells.Workingdilutionsmustbedeterminedbyenduser.BIOLOGicalInformationImmunogenHumansquamouscellcarcinomacells(SCC9)CloneASC-3ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.HostMouseSpecificityHumanbeta4integrin(CD104)IsotypeIgG1κSpeciesReactivityHumanAntibodyTypeMonoclonalAntibodyEntrezGeneNumberNM_000213.3NM_001005619.1NM_001005731.1EntrezGeneSummaryIntegrinsareheterodimerscomprisedofalphaandbetasubunits,thatarenoncovalentlyassociatedtransmembraneglycoproteinreceptors.Differentcombinationsofalphaandbetapolypeptidesformcomplexesthatvaryintheirligand-bindingspecificities.Integrinsmediatecell-matrixorcell-celladhesion,andtransducedsignalsthatregulategeneexpressionandcellgrowth.Thisgeneencodestheintegrinbeta4subunit,areceptorforthelaminins.Thissubunittendstoassociatewithalpha6subunitandislikelytoplayapivotalroleinthebiologyofinvasivecarcinoma.Mutationsinthisgeneareassociatedwithepidermolysisbullosawithpyloricatresia.Multiplealternativelysplicedtranscriptvariantsencodingdistinctisoformshavebeenfoundforthisgene.GeneSymbolITGB4CD104GP150UniProtNumberP16144UniProtSummaryFUNCTION:SwissProt:P16144#Integrinalpha-6/beta-4isareceptorforlaminin.Itplaysacriticalstructuralroleinthehemidesmosomeofepithelialcells.SIZE:1822aminoacids;202151DaSUBUNIT:Heterodimerofanalphaandabetasubunit.Beta-4associateswithalpha-6.SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.TISSUESPECIFICITY:Integrinalpha-6/beta-4ispredominantlyexpressedbyepithelia.Isoformbeta-4Disalsoexpressedincolonandplacenta.Isoformbeta-4Eisalsoexpressedinepidermis,lung,duodenum,heart,spleenandstomach.DOMAIN:SwissProt:P16144Thefibronectintype-III-likedomainsbindBPAG1andplectinandprobablyalsorecruitBP230.DISEASE:SwissProt:P16144#DefectsinITGB4areacauseofepidermolysisbullosaletaliswithpyloricatresia(EB-PA)[MIM:226730];alsoknownasjunctionalepidermolysisbullosawithpyloricatresia(PA-JEB)oraplasiacutiscongenitawithgastrointestinalatresia.EB-PAischaracterizedbymucocutaneousfragilityandgastrointestinalatresia,whichmostcommonlyaffectsthepylorus.&DefectsinITGB4areacauseofgeneralizedatrophicbenignepidermolysisbullosa(GABEB)[MIM:226650].Thisnonlethalformofjunctionalepidermolysisbullosaischaracterizedbylife-longblisteringoftheskin,associatedwithhairandtoothabnormalities.SIMILARITY:Belongstotheintegrinbetachainfamily.&Contains1Calx-betadomain.&Contains4fibronectintype-IIIdomains.&Contains1VWFAdomain.PhysicochemicalInformationDimensionsMaterialsInformationMaterialsInformation