M-MuLV Reverse Transcriptase is a DNA polymerase which utilizes RNA as a substrate and exhibits no measurable proofreading 3′→5′ exonuclease function. This enzyme can perform cDNA synthesis by extending off a DNA primer annealed to an RNA template, or can copy a single-stranded DNA template.Source of ProteinA recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.Supplied in50 mM Tris-HCl150 mM NaCl0.1 mM EDTA1 mM DTT0.1% NP-40 Alternative50% glycerolpH 7.6 @ 25°CSupplied WithB7040 (10X M MuLV RT Buffer)10X M-MuLV RT Buffer (B7040):500 mM Tris-HCl750 mM KCl30 mM MgCl2100 mM DTTpH 8.3 @ 25°CUnit Definition1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Enzymatics--核酸25 mM 100µmol dNTP Solution Mix--N2050L,N2050F
核酸25mM100µmoldNTPSolutionMix
摘要:四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
产品描述
Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.
四种天然脱氧核苷酸的等摩尔混合溶液(25mM)
SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5
产品信息
PartNumber N2050L,N2050F
Concentration 25mM
UnitSize 100µmol,20µmol
产品特性
StorageTemperature -25to-15°C
Purity ≥99%
BasePurity ≥99.5%
Pyrophosphate ≤0.003pmol/pmolofnucleotide
EnzScript™(MMLV逆转录酶,RNaseH-)
摘要:基于RNA的DNA聚合酶,无核酸内切酶活性
产品描述:
EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶,该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链cDNA以用于下游的一些用途,如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性,相比于野生型M-MLV逆转录酶,它支持全长转录物种产生更高的cDNA产量