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IBL/Interleukin-8 / IL-8 (monkey) ELISA/BE45581/

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BE45581

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Kitsize12x8MethodELISAIncubationtime1x2h,1x10minStandardrange19-1250U/mLSpecimen/Volumes50µLserum,plasma,cellculturesupernatantetal.Substrate/isotopeTMB450nmRegulatoryStatus:ForresearchuseonlyDetailsfor: Interleukin-8/IL-8(monkey)ELISAInterleukin-8selectivelystimulatestheABIlityofneutrophilsandT-lymphocytestoinvadeinjuredorinflamedtissue.IL-8alsoknownasCXCL8thatispurifiedfromperipheralbloodmonocytesisa10kDaproteinshowingatleast4variantforms(77,72,70,and69aminoacids,respectively)whichdifferinthelengthoftheN-terminalandreADIlyformdimersinsolution.Twocystin-bridgesareessentialfortheBIOLOGicalactivityofIL-8.ExogenousstimulilikeLPS,butalsoIL-1,TNF-alphaandTNF-betainducethesecretionofIL-8inavarietyofdifferentcelltypesincludingmonocytes,endothelialandepithelialcells,peripheralbloodmononuclearcells,dermalfibroblasts,keratinocytes,neutrophils,hepatocytes,synovialcells,andT-lymphocytes.TheactivitiesofIL-8arenotspecies-specific.HumanIL-8isalsoactiveinrodentandrabbitcellsanddiffersfromallothercytokinesinitsabilitytospecificallyactivateneutrophilgranulocytes.Thischemokineactivatesmultipleintracellularsignalingpathwaysdownstreamoftwocell-surface,Gprotein-coupledreceptors(CXCR1andCXCR2).ThepropertyofIL-8tostimulatemovementofneutrophilsacrossendothelialmonolayersinvitrosupportstheconceptofacentralroleforthismoleculeintheaccumulationofneutrophilsatinflammatorylesionsinvivo.Synonyms:Protein3-10C,CXCL8,NAP-1,GCP-1ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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