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Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
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产品说明
Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Usage Physical State: Freeze-dried solid Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.Conjugate Horseradish PeroxidaseHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.Images & References This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
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Jackson/AffiniPure Goat Anti-Alpaca IgG (H+L)/1.0 mg/128-005-003 | 128-005-003 | Normal Serums and Gamma Globulins | 其他二抗 | 95.00 | Jackson/AffiniPure Alpaca Anti-Human IgG (H+L)/1.0 mg/609-005-213 | 609-005-213 | Whole IgG Affinity-Purified Antibodies | 抗人 | 67.00 | Jackson/AffiniPure Alpaca Anti-Mouse IgG (H+L)/1.0 mg/615-005-214 | 615-005-214 | Whole IgG Affinity-Purified Antibodies | 抗小鼠 | 67.00 | Jackson/AffiniPure Alpaca Anti-Rabbit IgG (H+L)/1.0 mg/611-005-215 | 611-005-215 | Whole IgG Affinity-Purified Antibodies | 抗兔 | 67.00 | Jackson/Rhodamine Red™-X (RRX) AffiniPure Rabbit Anti-Rat IgG + IgM (H+L)/1.5 mg/312-295-044 | 312-295-044 | Whole IgG Affinity-Purified Antibodies | 抗大鼠 | 116.00 | Jackson/Alkaline Phosphatase IgG Fraction Monoclonal Mouse Anti-Fluorescein (FITC)/0.5 ml/200-052-037 | 200-052-037 | Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP | 抗荧光素 | 170.00 | Jackson/Bovine Serum Albumin (IgG-Free, Protease-Free)/250.0 g/001-000-173 | 001-000-173 | Bovine Serum Albumin (IgG-Free and Protease-Free) | 白蛋白 | 1044.00 | Jackson/ChromPure Goat IgG, whole molecule - Agarose/5.0 ml/005-000-052 | 005-000-052 | Immunoadsorbent Gels | 血清纯化蛋白 | 98.00 | Jackson/Normal Alpaca Serum/2.0 ml/028-000-001 | 028-000-001 | Normal Serums and Gamma Globulins | 封闭血清 | 33.00 | Jackson/Bovine Gamma Globulin/10.0 mg/001-000-002 | 001-000-002 | Normal Serums and Gamma Globulins | 球蛋白 | 53.00 | Jackson/Rabbit Anti-Bovine Whole Serum/2.0 ml/301-001-001 | 301-001-001 | Antisera | 抗牛 | 43.00 | Jackson/Donkey Anti-Chicken IgY (IgG) (H+L)/2.0 ml/703-001-003 | 703-001-003 | Antisera | 抗鸡 | 38.00 | Jackson/Rabbit Anti-Dog IgG (H+L)/2.0 ml/304-001-003 | 304-001-003 | Antisera | 抗犬 | 43.00 | Jackson/Rabbit Anti-Goat Whole Serum/2.0 ml/305-001-001 | 305-001-001 | Antisera | 抗山羊 | 43.00 | Jackson/Donkey Anti-Guinea Pig IgG (H+L)/2.0 ml/706-001-003 | 706-001-003 | Antisera | 抗豚鼠 | 38.00 | Jackson/Goat Anti-Horseradish Peroxidase/2.0 ml/123-001-021 | 123-001-021 | Antisera | 抗马 | 43.00 | Jackson/Rabbit Anti-Sheep IgG (H+L)/2.0 ml/313-001-003 | 313-001-003 | Antisera | 抗绵羊 | 43.00 | Jackson/AffiniPure Goat Anti-Cat IgG (H+L)/2.0 mg/102-005-003 | 102-005-003 | Whole IgG Affinity-Purified Antibodies | 抗猫 | 79.00 | Jackson/AffiniPure Goat Anti-Armenian Hamster IgG (H+L)/1.5 mg/127-005-099 | 127-005-099 | Whole IgG Affinity-Purified Antibodies | 抗仓鼠 | 83.00 | Jackson/AffiniPure Goat Anti-Swine IgG (H+L)/2.0 mg/114-005-003 | 114-005-003 | Whole IgG Affinity-Purified Antibodies | 抗猪 | 79.00 | Jackson/Peroxidase IgG Fraction Monoclonal Mouse Anti-Digoxin/0.5 ml/200-032-156 | 200-032-156 | Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP | 抗地高辛 | 169.00 | Jackson/Peroxidase IgG Fraction Monoclonal Mouse Anti-Biotin/0.5 ml/200-032-211 | 200-032-211 | Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP | 抗生物素 | 169.00 | Jackson/Goat Peroxidase-Anti-Peroxidase/0.25 ml/123-005-024 | 123-005-024 | Peroxidase-Anti-Peroxidase (PAP) | 抗过氧化物酶 | 83.00 | Jackson/Alexa Fluor® 680 Streptavidin/0.5 mg/016-620-084 | 016-620-084 | Streptavidin and Conjugates | 链霉亲和素结合物 | 116.00 |
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