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ADAR1抗体(ab88574)| Abcam中文官网
长期批量供应 —— 采用重组技术,可实现快速生产 首次实验即可成功 —— 经过大量验证确认了特异性 符合伦理标准 —— 产品不含动物成分 Recombinant full length protein within Human ADAR1 aa 1-1250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. 常规说明 The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As 存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. The Abpromise guarantee Abpromise™承诺保证使用ab88574于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。 IHC-PUse a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. 疾病相关 Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet. 序列相似性 Contains 1 A to I editase domain.Contains 2 DRADA repeats.Contains 3 DRBM (double-stranded RNA-binding) domains. 细胞定位 Cytoplasm. Nucleus nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus. 136 kDa double-stranded RNA-binding protein antibody 136kDa double stranded RNA binding protein antibody Adar 1 antibody Adenosine deaminase acting on RNA 1 A antibody Adenosine deaminase RNA specific 1 antibody Adenosine deaminase RNA specific antibody Adenosine deaminase that act on RNA antibody AGS6 antibody AV242451 antibody Double stranded RNA specific adenosine deaminase antibody Double-stranded RNA-specific adenosine deaminase antibody Double-stranded RNA-specific editase Adar antibody DRADA antibody Dsh antibody Dsrad antibody DSRAD_HUMAN antibody dsRNA adenosine deaminase antibody EC 3.5.4.- antibody G1P1 antibody IFI 4 antibody IFI-4 antibody IFI4 antibody Ifi4 protein antibody Interferon induced protein 4 antibody Interferon inducible protein 4 antibody Interferon-inducible protein 4 antibody K88DSRBP antibody mZaADAR antibody P136 antibody Pre-mRNA adenosine deaminase antibody RNA adenosine deaminase 1 antibody RNA-editing deaminase 1 antibody RNA-editing enzyme 1 antibody see all Anti-ADAR1 antibody (ab88574) at 1 µg/ml + Human liver lysate at 50 µgPredicted band size: 104 kDa Immunocytochemistry/ Immunofluorescence - Anti-ADAR1 antibody (ab88574)Image from Emmott E et al., J Proteome Res 9:5335-45 (2010). DOI: 10.1021/pr100593g; Fig 4.; October 1, 2010, Journal of Proteome Research with permission from the American Chemical Society. Immunofluorescence analysis of MOCK-infected HEK293T cells, staining ADAR1 (green) with ab88574.Cells were fixed in 4% formaldehyde and permeabilized with 0.2% Triton X-100 before incubating with fluorescent-conjugated anti-mouse secondary antibody. Nuclei were stained with DAPI (blue). ab88574 at 3 g/ml staining ADAR1 in formalin-fixed, paraffin-embedded Human lung tissue. Anti-ADAR1 antibody (ab88574) at 1 µg/ml + HepG2 cell lysate at 50 µgPredicted band size: 104 kDa 发表研究结果有使用 ab88574?请让我们知道,以便我们可以引用本数据表中的参考文章。 ab88574 被引用在 24 文献中. Shen Y et al. G protein-coupled oestrogen receptor promotes cell growth of non-small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling. J Cell Mol Med 25:284-296 (2021).PubMed: 33237585 Cai H et al. G3BP1 Inhibition Alleviates Intracellular Nucleic Acid-Induced Autoimmune Responses. J Immunol 206:2453-2467 (2021).PubMed: 33941659 Wu J et al. LINC01152 upregulates MAML2 expression to modulate the progression of glioblastoma multiforme via Notch signaling pathway. Cell Death Dis 12:115 (2021).PubMed: 33483471 Tang SJ et al. Cis- and trans-regulations of pre-mRNA splicing by RNA editing enzymes influence cancer development. Nat Commun 11:799 (2020).PubMed: 32034135 Vogel OA et al. The p150 Isoform of ADAR1 Blocks Sustained RLR signaling and Apoptosis during Influenza Virus Infection. PLoS Pathog 16:e1008842 (2020).PubMed: 32898178 View all Publications for this product Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) Concentration: 5% Temperature: 25 C The ADAR antibody ab88574 is raised against full-length human ADAR1, and it is a polyclonal antibody, which means it will potentially recognize all ADAR isoforms.Polyclonal antibodies are composed of many antibodies specific for different epitopes (amino acid sequences) on the immunogen (full-length ADAR, in this case). Monoclonal antibodies are specific for a single epitope. Read More Thank you for your enquiry.I am pleased to provide the following IHC-P protocol used for testing ab88574 . As discussed on the telephone, please note that this would be a guideline only and may require some further optimization.I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.Immunohistochemistry MethodDeparaffinize sections and rehydrate using PBS.Pre-treat (antigen retrieval) the sample with one of the following methods:No treatment at all. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125℃ for 4min and under 90℃ for 45min, cool sample subsequently.Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently. Place sample in 1X Tris/EDTA buffer (pH 9.0) and microwave at 750W for 20 minutes, cool sample subsequently. Place sample in HCl (2N) (pH 0.6˜0.9) at room temperature for 10˜20 minutes. Place sample in 0.1% trypsin and shake for 25 minutes at 37 C.Step-by-step procedure: 1. Incubate sections in 3% H2O2 in 1X PBS at room temperature for 10 minutes and then wash the sections again. 2. Incubate sections in blocking solution for 10 minutes. 3. Add primary antibodies (diluted in blocking solution) and incubate the sections overnight at 4 C, wash sample with 1X PBS afterwards. 4. Incubate sections with labeled polymer for 30 min followed by washing the sections with PBS. 5. Application of substrate solution (DAB or other suitable peroxidase substrate). Wash sample thoroughly under running tap water. 6. Counter stain the samples in Mayer s hematoxylin. 7. Dehydrate and mount samples.Reagents:Blocking solution or Antibody Diluent (commercially purchased) Immunodection Kit- EnVision Detection Kit, Peroxidase/DAB,Rabbit/Mouse Citrate buffer, pH 6.0: 10 mM sodium citrate buffer 1X Tris/EDTA, pH 9.0: 10 mM Tris base, 1 mM EDTA solution, 0.05% Tween 20 HCl solution (2N), pH 0.6˜0.9: prepared in distilled water Read More Thank you for contacting Abcam about this issue. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. Before I may issue a free of charge replacement, I would appreciate it if you could send me the specific of the protocols that you had used with Ab97640 for our records. The details provided enable us to closely monitor the quality of our products.   For your Western blotting protocol could you please let me know about your blocking step such as what you blocked in, for how long and at what temperature? Could you also inform us as to the incubation conditions for the primary and secondary antibodies? What concentrations these were used at, as well as any adustments you made while troubleshooting? Where you able to run an isotype control? For your ICC-IF could you give me the same information as well as antigen retrieval steps you may have used? Thank you very much for your cooperation. If you have any questions please feel free to contact us. Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com