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Signagen/GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells/SL100489-OS/0.1 mL
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Description GenJet™ DNA In Vitro Transfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-). Refer to the following optimal transfection conditions for maximal transfection efficiency on U2 OS cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates. Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) 60~70% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes No 48 hours EGFP pEGFP-N3 (CMV promoter) 95% Storage Condition Store at 4 °C. If stored properly, the product is stable for 12 months or longer A Picture Showing Transfection Efficiency of GenJet™ Reagent on U2 OS Cells GenJet™ reagent is optimized for U2OS. U2OS cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection Data Sheet

SignaGen生物技术公司于2006年成立于美国马里兰州,主要专注于各种转染试剂的研究与开发。公司的创始人系几个来自美国国立卫生研究院(National Institutes of Health, NIH)的致力于生物医学研究的科学家。自SignaGen生物技术公司建立的第一天起,他们就一直致力于成为美国基因转导及基因治疗领域的先驱。目前,SignaGen公司已经成功地开发出三大系列分别用于体内和体外的DNA和RNA转染试剂,是目前世界上唯一拥有从多聚阳离子聚合物到阳离子型脂质体转染试剂的生物公司。

当公司的创始人们还在NIH从事生物医学基础研究时,他们就经常需要将外源性基因导入到哺乳动物细胞。他们一方面惊讶于DNA转染试剂出色的转染效率,另一方面他们也惊讶于转染试剂昂贵的价格。从那一刻起,他们开始尝试着用老掉牙的旋转蒸发仪在自己的实验室制作基于脂质体的DNA转染试剂。经过耗时费力的程序及制剂的优化工作,他们自制的DNA转染试剂同市场上最好的转染试剂一样出色,但是成本只是这些商用转染试剂的零头。从那时起,他们开始质疑这些商用转染试剂高得离谱的价格,他们为什么不可以自己制作和销售这些转染试剂?他们为什么不可以为他们的同行们提供高效率,低价位的转染试剂呢?基于这一想法,他们成立了该公司并开始研制开发DNA和RNA转染试剂。

2007年1月,他们向市场投放第一个DNA转染试剂——GenJet™体外DNA转染试剂。该试剂因为极高的转染效率和极低的毒性迅速获得生物医学科研工作者的青睐。接下来他们迅速地升级了该转染试剂,先后研制出了GenJet™(版本II)和GenJet™ Plus等系列高级升级产品。这些升级产品因为显著地增加了DNA结合基团,所以转染效率更高。

最近他们又先后研发成功了基于多聚阳离子聚合物的转染试剂——PolyJet™体外DNA转染试剂和基于脂质体的LipoD293™体外DNA转染试剂。PolyJet™体外DNA转染试剂是一种新型的生物可降解的聚合物,进入细胞后,该聚合物迅速完全地降解,从而几乎不产生细胞毒性;LipoD293™体外DNA转染试剂则为一个加强型DNA转染试剂,因为其中添加了多肽增强剂,所以其转染效率非常高,最适用于难以转染的哺乳动物细胞。目前,SignaGen转染试剂已被超过169篇国际文献成功引用,获得了广大科研工作者的一致好评。