首页 > Usbio > Usbio/EIF1, Recombinant, Human (Eukaryotic Translation Initiation Factor 1, A121, EIF-1, EIF1A, ISO1, SUI1)/100ug/E8947-95

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Usbio/EIF1, Recombinant, Human (Eukaryotic Translation Initiation Factor 1, A121, EIF-1, EIF1A, ISO1, SUI1)/100ug/E8947-95

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EIF1, also known as eukaryotic translation initiation factor 1, is a universally conserved translation factor that is necessary for scanning and involved in initiation site selection. This protein promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. ||Recombinant human EIF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Endotoxin: ≤1EU/1ug (LAL).||Amino Acid Sequence:|MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMSA IQNLHSFDPF ADASKGDDLL PAGTEDYIHI RIQQRNGRKT LTTVQGIADD YDKKKLVKAF KKKFACNGTV IEHPEYGEVI QLQGDQRKNI CQFLVEIGLA KDDQLKVHGF||Molecular Weight: 16.9kD (150aa)||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Source: E.coli|Purity: ~90% by SDS-PAGE. |Concentration: ~0.5mg/ml |Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 1mM DTT, 0.1M NaCl, 10% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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