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MTI-GlobalStem/Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients./1.0 ug DNA/20-abx321927
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Oligonucleotide primers based mostly on Borrelia burgdorferi sensu lato ospA gene sequences have been designed to be used within the PCR to kind all (SL primers) or every (GI to GIII primers) of the B. burgdorferi sensu lato genospecies concerned in Lyme illness. These genospecies-specific primers have been then used within the PCR on 24 organic fluids collected from 18 neuroborreliosis sufferers.Among the many samples examined, 20 contained DNA from Borrelia garinii, 11 contained DNA from B. burgdorferi sensu stricto, and 10 contained DNA from Borrelia afzelii. In toto, 10 sufferers appeared to have been contaminated by a single genospecies and eight have been contaminated by a couple of Lyme disease-associated genospecies. Serum specimens from six sufferers have been absorbed with heterologous antigens and examined by Western blotting (immunoblotting).In 4 instances, residual immunodetection revealed particular epitopes of genospecies additionally detected by PCR; in two of them, the concordant outcomes indicated pluri-infection of the sufferers. Within the different two instances, Western blotting confirmed particular antibodies for 2 genospecies of Borrelia, whereas PCR detected DNA from just one. In abstract, the information underscored the comparatively excessive prevalence of pluri-infections in Lyme illness and confirmed the affiliation of B. garinii with neuroborreliosis.Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells.Human embryonic stem cells (hESCs) have nice potential to be used in analysis and regenerative medication, however little or no is understood concerning the elements that keep these cells within the pluripotent state. We investigated the position of three main mitogenic brokers current in serum–sphingosine-1-phosphate (S1P), lysophosphatidic acid (LPA), and platelet-derived progress issue (PDGF)–in sustaining hESCs.EEF1B2 antibody70R-49683Fitzgerald100 ulEUR 244.00Description: Purified Polyclonal EEF1B2 antibodyEEF1B2 antibody70R-2143Fitzgerald50 ugEUR 467.00Description: Rabbit polyclonal EEF1B2 antibody raised against the middle region of EEF1B2EEF1B2 Antibody1-CSB-PA004634CusabioEUR 222.00EUR 195.00100ug50ugForm: Liquid Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Description: A polyclonal antibody against EEF1B2. Recognizes EEF1B2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000EEF1B2 Antibody1-CSB-PA335050DSR1HUCusabioEUR 222.00EUR 335.00100ul50ulForm: Liquid Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Antigen Affinity PurifiedDescription: A polyclonal antibody against EEF1B2. Recognizes EEF1B2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200EEF1B2 Antibody1-CSB-PA335050DSR2HUCusabioEUR 222.00EUR 335.00100ul50ulForm: Liquid Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Antigen Affinity PurifiedDescription: A polyclonal antibody against EEF1B2. Recognizes EEF1B2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200EEF1B2 Antibody43073-100ulSAB100ulEUR 252.00EEF1B2 antibody39022-100ulSAB100ulEUR 252.00EEF1B2 cloning plasmidCSB-CL335050HU1-10ugCusabio10ugEUR 233.00Formulation: 10 μg plasmid + 200μl Glycerol Length: 678 Sequence: atgggtttcggagacctgaaaagccctgccggcctccaggtgctcaacgattacctggcggacaagagctacatcgaggggtatgtgccatcacaagcagatgtggcagtatttgaagccgtgtccagcccaccgcctgccgacttgtgtcatgccctacgttggtataatcacatShow moreDescription: A cloning plasmid for the EEF1B2 gene.EEF1B2 cloning plasmidCSB-CL335050HU2-10ugCusabio10ugEUR 233.00Formulation: 10 μg plasmid + 200μl Glycerol Length: 678 Sequence: atgggtttcggagacctgaaaagccctgccggcctccaggtgctcaacgattacctggcggacaagagctacatcgaggggtatgtgccatcacaagcagatgtggcagtatttgaagccgtgtccagcccaccgcctgccgacttgtgtcatgccctacgttggtataatcacatShow moreDescription: A cloning plasmid for the EEF1B2 gene.EEF1B2 Blocking Peptide33R-9624Fitzgerald100 ugEUR 180.00Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EEF1B2 antibody, catalog no. 70R-2143EEF1B2 Blocking Peptide20-abx062558AbbexaEUR 272.00EUR 411.001 mg5 mgShipped within 5-10 working days.EEF1B2 Conjugated AntibodyC39022SAB100ulEUR 397.00EEF1B2 Conjugated AntibodyC43073SAB100ulEUR 397.00Anti-EEF1B2 antibodyPAab02643Lifescience Market100 ugEUR 355.00Anti-EEF1B2 antibodyPAab02644Lifescience Market100 ugEUR 355.00anti- EEF1B2 antibodyFNab02643FN Test100µgEUR 505.25Recommended dilution: WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200Immunogen: eukaryotic translation elongation factor 1 beta 2Uniprot ID: P24534Gene ID: 1933Research Area: Cancer, MetabolismDescription: Antibody raised against EEF1B2anti- EEF1B2 antibodyFNab02644FN Test100µgEUR 505.25Immunogen: eukaryotic translation elongation factor 1 beta 2Uniprot ID: P24534Gene ID: 1933Research Area: Cancer, MetabolismDescription: Antibody raised against EEF1B2anti- EEF1B2 antibodyFNab02645FN Test100µgEUR 585.00Recommended dilution: WB: 1:1000-1:4000 IF: 1:50-1:500Immunogen: eukaryotic translation elongation factor 1 beta 2Uniprot ID: P24534Gene ID: 1933Research Area: Cancer, MetabolismDescription: Antibody raised against EEF1B2Anti-EEF1B2 antibodySTJ28663St John"s Laboratory100 µlEUR 413.00Description: This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5" UTR.Anti-eEF1B2(3A5)YF-MA12778Abfrontier100 ugEUR 363.00Description: Mouse monoclonal to eEF1B2Human EEF1B2 shRNA Plasmid20-abx951334AbbexaEUR 801.00EUR 1121.00150 µg300 µgShipped within 15-20 working days.EEF1B2 protein (His tag)80R-1704Fitzgerald10 ugEUR 305.00Description: Purified recombinant Human EEF1B2 proteinEEF1B2 ELISA KIT|HumanEF009297Lifescience Market96 TestsEUR 689.00EEF1B2 Recombinant Protein (Human)RP010198ABM100 ugAsk for priceEEF1B2 Recombinant Protein (Human)RP010201ABM100 ugAsk for priceEEF1B2 Recombinant Protein (Rat)RP199085ABM100 ugAsk for priceEEF1B2 Recombinant Protein (Mouse)RP130901ABM100 ugAsk for price[KO Validated] EEF1B2 Rabbit pAbA6580-100ulAbclonal100 ulEUR 410.00[KO Validated] EEF1B2 Rabbit pAbA6580-200ulAbclonal200 ulEUR 571.00[KO Validated] EEF1B2 Rabbit pAbA6580-20ulAbclonal20 ulEUR 221.00[KO Validated] EEF1B2 Rabbit pAbA6580-50ulAbclonal50 ulEUR 287.00EEF1B2 ORF Vector (Human) (pORF)ORF003400ABM1.0 ug DNAEUR 95.00EEF1B2 ORF Vector (Human) (pORF)ORF003401ABM1.0 ug DNAEUR 95.00Eef1b2 ORF Vector (Rat) (pORF)ORF066363ABM1.0 ug DNAEUR 506.00Eef1b2 ORF Vector (Mouse) (pORF)ORF043635ABM1.0 ug DNAEUR 506.00Human Elongation factor 1-beta (EEF1B2)1-CSB-EP335050HUCusabioEUR 380.00EUR 214.00EUR 1309.00EUR 560.00EUR 873.00EUR 262.00100ug10ug1MG200ug500ug50ugMW: 51.6 kDa Buffer composition: Tris-based buffer with 50% glycerol.Description: Recombinant Human Elongation factor 1-beta(EEF1B2) expressed in E.coliEEF1B2 sgRNA CRISPR Lentivector set (Human)K0656701ABM3 x 1.0 ugEUR 339.00Eef1b2 sgRNA CRISPR Lentivector set (Mouse)K4892201ABM3 x 1.0 ugEUR 339.00Eef1b2 sgRNA CRISPR Lentivector set (Rat)K6406801ABM3 x 1.0 ugEUR 339.00EEF1B2 Protein Vector (Human) (pPB-C-His)PV013597ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPB-N-His)PV013598ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPM-C-HA)PV013599ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPM-C-His)PV013600ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPB-C-His)PV013601ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPB-N-His)PV013602ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPM-C-HA)PV013603ABM500 ngEUR 329.00EEF1B2 Protein Vector (Human) (pPM-C-His)PV013604ABM500 ngEUR 329.00EEF1B2 sgRNA CRISPR Lentivector (Human) (Target 1)K0656702ABM1.0 ug DNAEUR 154.00EEF1B2 sgRNA CRISPR Lentivector (Human) (Target 2)K0656703ABM1.0 ug DNAEUR 154.00EEF1B2 sgRNA CRISPR Lentivector (Human) (Target 3)K0656704ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Mouse) (Target 1)K4892202ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Mouse) (Target 2)K4892203ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Mouse) (Target 3)K4892204ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Rat) (Target 1)K6406802ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Rat) (Target 2)K6406803ABM1.0 ug DNAEUR 154.00Eef1b2 sgRNA CRISPR Lentivector (Rat) (Target 3)K6406804ABM1.0 ug DNAEUR 154.00EEF1B2 Protein Vector (Mouse) (pPB-C-His)PV174538ABM500 ngEUR 603.00EEF1B2 Protein Vector (Mouse) (pPB-N-His)PV174539ABM500 ngEUR 603.00EEF1B2 Protein Vector (Mouse) (pPM-C-HA)PV174540ABM500 ngEUR 603.00EEF1B2 Protein Vector (Mouse) (pPM-C-His)PV174541ABM500 ngEUR 603.00

MTI-GlobalStem公司成立于2006年,最初名为GlobalStem,在2010年被Molecular Transfer公司(MTI)收购,总部位于美国马里兰州盖瑟斯堡,致力于开发和推广细胞转染、神经生物学以及干细胞研究所用的工具和试剂。此外,这家公司还提供干细胞、分化神经元和大规模的细胞培养制造服务。

MTI团队的科学家也并非等闲之辈,他们参与开发了转染、转化、核酸技术以及基因表达领域的多个知名产品。据介绍,这些科学家在BRL/Life Technologies任职期间曾领导著名的转染试剂Lipofectamine的开发。他们还与加州大学旧金山分校的Doug Hanahan教授合作,开发并推广了赫赫有名的DH5α?和DH10B?系列。

如今,MTI-GlobalStem凭借在转染、神经生物学和干细胞生物学方面的专长,开发出一系列优势产品。他们推出了原代和ipsC来源的神经细胞、干细胞和滋养层细胞,并开发出新型的转染试剂,能够以高效率将核酸导入干细胞和神经元。针对目前火热的CRISPR基因编辑,MTI-GlobalStem也推出了一系列导入试剂。