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MTI-GlobalStem/Synapse formation and establishment of neuronal polarity by P19 embryonic carcinoma cells and embryonic stem cells./3 x 1.0 ug/20-abx109748
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Various totally different cell strains that exhibit a partial neuronal phenotype have been recognized, however in lots of circumstances the complete extent of their neuronal differentiation has not been instantly addressed by useful research. We have now used electrophysiology and immunofluorescence to look at the formation of synapses and the event of neuronal polarity by murine embryonic stem (ES) cells and the mouse P19 embryonic carcinoma cell line.Inside 2-Three weeks after induction by retinoic acid, subsets of P19 and ES cells shaped excitatory synapses, mediated by glutamate receptors, or inhibitory synapses, mediated by receptors for GABA or glycine. In ES-cell cultures, each NMDA and non-NMDA receptors contributed to the excitatory postsynaptic response.EEF1D siRNA20-abx914980AbbexaEUR 551.00EUR 732.0015 nmol30 nmolShipped within 5-10 working days.EEF1D siRNA20-abx914981AbbexaEUR 551.00EUR 732.0015 nmol30 nmolShipped within 5-10 working days.EEF1D siRNA20-abx901644AbbexaEUR 551.00EUR 732.0015 nmol30 nmolShipped within 5-10 working days.EEF1D antibody70R-17002Fitzgerald50 ulEUR 435.00Description: Rabbit polyclonal EEF1D antibodyEEF1D AntibodyABD6974Lifescience Market100 ugEUR 438.00EEF1D Antibody1-CSB-PA007431GA01HUCusabioEUR 597.00EUR 333.00150ul50ulForm: Liquid Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity PurifiedDescription: A polyclonal antibody against EEF1D. Recognizes EEF1D from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHCEEF1D Antibody1-CSB-PA007431LA01HUCusabioEUR 317.00EUR 335.00100ug50ugForm: Liquid Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purifiedDescription: A polyclonal antibody against EEF1D. Recognizes EEF1D from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000EEF1D AntibodyDF6974Affbiotech200ulEUR 304.00Description: EEF1D Antibody detects endogenous levels of total EEF1D.EEF1D antibody38412-100ulSAB100ulEUR 252.00anti-EEF1DYF-PA11496Abfrontier50 ugEUR 363.00Description: Mouse polyclonal to EEF1Danti-EEF1DYF-PA11497Abfrontier100 ugEUR 403.00Description: Rabbit polyclonal to EEF1Danti-EEF1DYF-PA23623Abfrontier50 ulEUR 334.00Description: Mouse polyclonal to EEF1DEEF1D Polyclonal AntibodyA54224EpiGentek100 µgEUR 570.55Description: Ask the seller for detailsEEF1D Rabbit pAbA2509-100ulAbclonal100 ulEUR 308.00EEF1D Rabbit pAbA2509-200ulAbclonal200 ulEUR 459.00EEF1D Rabbit pAbA2509-20ulAbclonal20 ulEUR 183.00EEF1D Rabbit pAbA2509-50ulAbclonal50 ulEUR 223.00EEF1D Blocking PeptideDF6974-BPAffbiotech1mgEUR 195.00Polyclonal EEF1D AntibodyAMM07014GLeading Biology0.05mgEUR 484.00Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EEF1D . This antibody is tested and proven to work in the following applications:EEF1D Conjugated AntibodyC38412SAB100ulEUR 397.00EEF1D cloning plasmidCSB-CL007431HU1-10ugCusabio10ugEUR 654.00Formulation: 10 μg plasmid + 200μl Glycerol Length: 1944 Sequence: atgaggagcgggaaggcctcctgcaccctggagaccgtgtgggaagacaagcacaagtatgaggaggccgagcggcgcttctacgaacacgaggccacacaggcggccgcctccgcccagcagctgccagccgaggggccagccatgaatgggcccggccaggacgaccctgaggShow moreDescription: A cloning plasmid for the EEF1D gene.EEF1D cloning plasmidCSB-CL007431HU2-10ugCusabio10ugEUR 233.00Formulation: 10 μg plasmid + 200μl Glycerol Length: 846 Sequence: atggctacaaacttcctagcacatgagaagatctggttcgacaagttcaaatatgacgacgcagaaaggagattctacgagcagatgaacgggcctgtggcaggtgcctcccgccaggagaacggcgccagcgtgatcctccgtgacattgcgagagccagagagaacatccagaaShow moreDescription: A cloning plasmid for the EEF1D gene.Anti-EEF1D antibodyPAab02646Lifescience Market100 ugEUR 355.00anti- EEF1D antibodyFNab02646FN Test100µgEUR 505.25Recommended dilution: WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200Immunogen: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)Uniprot ID: P29692Gene ID: 1936Research Area: Signal Transduction, MetabShow moreDescription: Antibody raised against EEF1Danti- EEF1D antibodyFNab02647FN Test100µgEUR 585.00Recommended dilution: WB: 1:500-1:2000 IF: 1:10-1:100 IHC: 1:50-1:500Immunogen: eukaryotic translation elongation factor 1 delta(guanine nucleotide exchange protein)Uniprot ID: P29692Gene ID: 1936Research Area: Signal Transduction, MetabolismDescription: Antibody raised against EEF1Danti- EEF1D antibodyFNab02648FN Test100µgEUR 548.75Recommended dilution: WB: 1:500-1:2000 IHC: 1:20-1:200Immunogen: eukaryotic translation elongation factor 1 delta(guanine nucleotide exchange protein)Uniprot ID: P29692Gene ID: 1936Research Area: Signal Transduction, MetabolismDescription: Antibody raised against EEF1DAnti-EEF1D antibodySTJ23477St John"s Laboratory100 µlEUR 277.00Description: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.Anti-EEF1D(4B12)YF-MA10264Abfrontier100 ugEUR 363.00Description: Mouse monoclonal to EEF1DHuman EEF1D shRNA Plasmid20-abx951335AbbexaEUR 801.00EUR 1121.00150 µg300 µgShipped within 15-20 working days.Mouse EEF1D shRNA Plasmid20-abx975725AbbexaEUR 801.00EUR 1121.00150 µg300 µgShipped within 15-20 working days.EEF1D protein (His tag)80R-1713Fitzgerald50 ugEUR 397.00Description: Purified recombinant Human EEF1D proteinEEF1D Antibody, HRP conjugated1-CSB-PA007431LB01HUCusabioEUR 317.00EUR 335.00100ug50ugForm: Liquid Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purifiedDescription: A polyclonal antibody against EEF1D. Recognizes EEF1D from Human. This antibody is HRP conjugated. Tested in the following application: ELISAEEF1D Antibody, FITC conjugated1-CSB-PA007431LC01HUCusabioEUR 317.00EUR 335.00100ug50ugForm: Liquid Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purifiedDescription: A polyclonal antibody against EEF1D. Recognizes EEF1D from Human. This antibody is FITC conjugated. Tested in the following application: ELISAEEF1D Antibody, Biotin conjugated1-CSB-PA007431LD01HUCusabioEUR 317.00EUR 335.00100ug50ugForm: Liquid Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purifiedDescription: A polyclonal antibody against EEF1D. Recognizes EEF1D from Human. This antibody is Biotin conjugated. Tested in the following application: ELISARat EEF1D shRNA Plasmid20-abx989020AbbexaEUR 801.00EUR 1121.00150 µg300 µgShipped within 15-20 working days.EEF1D ELISA KIT|HumanEF009298Lifescience Market96 TestsEUR 689.00EEF1D Recombinant Protein (Human)RP010204ABM100 ugAsk for priceEEF1D Recombinant Protein (Human)RP010207ABM100 ugAsk for priceEEF1D Recombinant Protein (Rat)RP199088ABM100 ugAsk for priceEEF1D Recombinant Protein (Mouse)RP130904ABM100 ugAsk for priceEEF1D Recombinant Protein (Mouse)RP130907ABM100 ugAsk for priceEEF1D Polyclonal Antibody, Biotin ConjugatedA54221EpiGentek100 µgEUR 570.55Description: reagents widely citedEEF1D Polyclonal Antibody, FITC ConjugatedA54222EpiGentek100 µgEUR 570.55Description: Ask the seller for detailsEEF1D Polyclonal Antibody, HRP ConjugatedA54223EpiGentek100 µgEUR 570.55Description: The best epigenetics productsPolyclonal EEF1D Antibody (N-term)AMM07016GLeading Biology0.1mlEUR 484.00Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EEF1D (N-term). This antibody is tested and proven to work in the following applications:EEF1D ORF Vector (Human) (pORF)ORF003402ABM1.0 ug DNAEUR 95.00EEF1D ORF Vector (Human) (pORF)ORF003403ABM1.0 ug DNAEUR 95.00Eef1d ORF Vector (Rat) (pORF)ORF066364ABM1.0 ug DNAEUR 506.00Eef1d ORF Vector (Mouse) (pORF)ORF043636ABM1.0 ug DNAEUR 506.00Eef1d ORF Vector (Mouse) (pORF)ORF043637ABM1.0 ug DNAEUR 506.00Human Elongation factor 1-delta (EEF1D)1-CSB-EP007431HUCusabioEUR 380.00EUR 214.00EUR 1309.00EUR 560.00EUR 873.00EUR 262.00100ug10ug1MG200ug500ug50ugMW: 35 kDa Buffer composition: Tris-based buffer with 50% glycerol.Description: Recombinant Human Elongation factor 1-delta(EEF1D) expressed in E.coliEEF1D sgRNA CRISPR Lentivector set (Human)K0657001ABM3 x 1.0 ugEUR 339.00Eef1d sgRNA CRISPR Lentivector set (Mouse)K4608201ABM3 x 1.0 ugEUR 339.00Eef1d sgRNA CRISPR Lentivector set (Rat)K7151201ABM3 x 1.0 ugEUR 339.00Monoclonal EEF1D Antibody (monoclonal) (M04), Clone: 4B12AMM07015GLeading Biology0.1mgEUR 484.00Description: A Monoclonal antibody against Human EEF1D (monoclonal) (M04). The antibodies are raised in mouse and are from clone 4B12. This antibody is applicable in WB and IF, EEEF1D Protein Vector (Human) (pPB-C-His)PV013605ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPB-N-His)PV013606ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPM-C-HA)PV013607ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPM-C-His)PV013608ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPB-C-His)PV013609ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPB-N-His)PV013610ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPM-C-HA)PV013611ABM500 ngEUR 329.00EEF1D Protein Vector (Human) (pPM-C-His)PV013612ABM500 ngEUR 329.00EEF1D sgRNA CRISPR Lentivector (Human) (Target 1)K0657002ABM1.0 ug DNAEUR 154.00EEF1D sgRNA CRISPR Lentivector (Human) (Target 2)K0657003ABM1.0 ug DNAEUR 154.00EEF1D sgRNA CRISPR Lentivector (Human) (Target 3)K0657004ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Mouse) (Target 1)K4608202ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Mouse) (Target 2)K4608203ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Mouse) (Target 3)K4608204ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Rat) (Target 1)K7151202ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Rat) (Target 2)K7151203ABM1.0 ug DNAEUR 154.00Eef1d sgRNA CRISPR Lentivector (Rat) (Target 3)K7151204ABM1.0 ug DNAEUR 154.00EEF1D Protein Vector (Mouse) (pPB-C-His)PV174542ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPB-N-His)PV174543ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPM-C-HA)PV174544ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPM-C-His)PV174545ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPB-C-His)PV174546ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPB-N-His)PV174547ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPM-C-HA)PV174548ABM500 ngEUR 603.00EEF1D Protein Vector (Mouse) (pPM-C-His)PV174549ABM500 ngEUR 603.00

MTI-GlobalStem公司成立于2006年,最初名为GlobalStem,在2010年被Molecular Transfer公司(MTI)收购,总部位于美国马里兰州盖瑟斯堡,致力于开发和推广细胞转染、神经生物学以及干细胞研究所用的工具和试剂。此外,这家公司还提供干细胞、分化神经元和大规模的细胞培养制造服务。

MTI团队的科学家也并非等闲之辈,他们参与开发了转染、转化、核酸技术以及基因表达领域的多个知名产品。据介绍,这些科学家在BRL/Life Technologies任职期间曾领导著名的转染试剂Lipofectamine的开发。他们还与加州大学旧金山分校的Doug Hanahan教授合作,开发并推广了赫赫有名的DH5α?和DH10B?系列。

如今,MTI-GlobalStem凭借在转染、神经生物学和干细胞生物学方面的专长,开发出一系列优势产品。他们推出了原代和ipsC来源的神经细胞、干细胞和滋养层细胞,并开发出新型的转染试剂,能够以高效率将核酸导入干细胞和神经元。针对目前火热的CRISPR基因编辑,MTI-GlobalStem也推出了一系列导入试剂。