首页 > BPS Bioscience > Bpsbioscience/PDE7B-HEK293 Recombinant Cell Line/60412/2 vials

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Bpsbioscience/PDE7B-HEK293 Recombinant Cell Line/60412/2 vials

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60412

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4000-520-616

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Species:HumanHostSpecies:HEK293(human)Genbank#:NM_018945Background:Phosphodiesterases(PDEs)regulatetheintracellularlevelsofcAMPandcGMPbyhydrolyzingcAMPandcGMPtotheirinactive5"monophosphates.Thesecyclicnucleotidesplayanimportantroleassecondmessengersindiversephysiologicalfunctions.PDE7isacAMP-specificenzymeandtwoPDEgenes(PDE7AandPDE7B)havebeenidentified.PDE7iswidelyexpressedinvarioustissues,withPDE7Afoundprimarilyinskeletalmuscle,Tlymphocytes,andpancreas,whilehighlevelsofPDE7Baredetectedinbrain,heart,andliver.InhibitionofPDE7activitybyitsinhibitorsleadstoelevatedintracellularlevelofcAMP.Description:RecombinantHEK293celllineexpressinghumanPDE7B(phosphodiesterase7B,accessionnumberNM_018945).HumanPDE7Bhasbeenstablyexpressedinahumanembryonickidney(HEK293)cellline.PDE7BexpressionwasconfirmedbyWesternblotting.ThefunctionofexpressedPDE7BwasvalidatedbycAMPdetectionassay.TheregulationofintracellularlevelofcAMPbyPDE7BinPDE7Bstably-expressedHEK293cellswascharacterizedbyacell-basedreporterassayusingpCRE-lucreportervector.pCRE-luccontainstheluciferasegenethatisunderthecontrolofcAMPresponseelement(CRE).ElevationofintracellularcAMPactivatescAMPresponseelementbindingprotein(CREB)tobindCREandinducestheexpressionofluciferase.WhencellstransientlytransfectedwithpCRE-lucreporterwereactivatedbyforskolin,thelevelofcAMPwasupregulatedinparentalHEK293cellsinducingtheexpressionoftheluciferasereporterwhereashPDE7B-HEK293cellsshowedreductioninthelevelofcAMPthatresultedinloweredexpressionofluciferase.UniProtQ9NP56Synonym(s):PDE7B,phosphodiesterase7BSuppliedAs:Eachvialcontains~1X106cellsin1mlof10%DMSO.MycoplasmaTesting:ThecelllinehasbeenscreenedusingthePCR-basedVenorGeMMycoplasmaDetectionkit(SigmaAldrich)toconfirmtheabsenceofMycoplasmaspecies.Format:aqueoussolutioncontainingDMSOInstructionsforuse:Seedatasheetfordetailedculturingandassayprotocol.Storage/StABIlity:Storeinliquidnitrogenimmediatelyuponreceipt.Application(s):UsefultomonitorcAMPandPDE7BsignalingpathwayactivityandtoscreenactivatorsorinhibitorsofPDE7B.Reference(s):1.Malik,R.etal.(2008)Cloning,stableexpressionofhumanphosphodiesterase7AanddevelopmentofanassayforscreeningofPDE7selectiveinhibitors.Appl.Microbiol.Biotechnol.77(5):1167-11732.FanChung,K.(2006)Phosphodiesteraseinhibitorsinairwaysdisease.Eur.J.Pharmacol.533(1-3):110-117Notes:Contactusforpricing.LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.Warning(s):Avoidfreeze/thawcyclesScientificCategory:PDEProductType:CellLineDatashownislot-specific.Contactusforspecificinformationonotherlots.

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