首页 > BPS Bioscience > Bpsbioscience/PDE4D Cell-Based Reporter Assay Kit/60505/500 reactions

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Bpsbioscience/PDE4D Cell-Based Reporter Assay Kit/60505/500 reactions

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Description:ThePDE4Dcell-basedactivityassayisdesignedforscreeninginhibitorsofPDE4D7inculturedcells.TheassayisbasedontransfectingcellswiththeCREluciferasereporter.CREreportercontainsthefireflyluciferasegeneunderthecontrolofcAMPresponseelement(CRE).ElevationofintracellularcAMPactivatesCREbindingprotein(CREB)tobindCREandinducetheexpressionofluciferase.WhencellstransientlytransfectedwithCREreporterareactivatedbyforskolin,theintracellularlevelofcAMPisupregulated,whichinducestheexpressionofCREluciferasereporter.However,whencellsareco-transfectedwithPDE4D7expressionvectorandCREreporter,thelevelofforskolin-inducedcAMPisreduced,resultinginlowerexpressionlevelofluciferase.WhencellsaretreatedwithPDE4DinhibitortoinhibitPDE4D7activity,cAMPlevelisrestored,resultinginhigherluciferaseactivity.Synonym(s):PDE4D,phosphodiesterase4D,PDE4DCell-BasedActivityAssayKitSuppliedAs:ThekitincludesCREluciferasereporter(premixedwithconstitutively-expressingRenilla(seapansy)luciferasevectorthatservesasaninternalcontrolfortransfectionefficiency),PDE4D7expressionvector,andforskolin.Format:Instructionsforuse:Seeassaykitdatasheetfordetailedprotocol.Storage/StABIlity:Stableatleast6monthsfromdateofreceipt,whenstoredasdirected(-20°C)Application(s):ThekitisdesignedforscreeninginhibitorsofPDE4DandformonitoringPDE4D/cAMPsignalingpathwayactivity.Reference(s):1.MontminyMRetal.(1987).Nature328(6126):175-1782.FanChung,K.(2006).Eur.J.Pharmacol.533(1-3):110-1173.Malik,R.etal.(2008).Appl.Microbiol.Biotechnol.77(5):1167-1173Notes:Thevectorsaresuitablefortransienttransfection.TheyareNOTsuitablefortransformationandamplificationinbacteria.Warning(s):Avoidfreeze/thawcyclesScientificCategory:PDEProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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