首页 > BPS Bioscience > Bpsbioscience/BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit/72008/96 reactions

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Bpsbioscience/BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit/72008/96 reactions

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Background:ThebindingofBTLAtotheHVEMreceptor(herpesvirusentrymediator,alsoknownasTNFRSF14)triggerscellsignalingpathwaysinvolvedinthenegativeregulationofTcells.BTLA:HVEMinteractionplaysakeyroleintheregulationofinflammatory,autoimmune,andantitumorresponses,andisanimportanttargetfordrugdiscovery.Description:TheBTLA:HVEM[Biotinylated]InhibitorScreeningAssayKitisdesignedforscreeningandprofilinginhibitorsofBTLA:HVEMsignaling.Thekeytothiskitisthehighsensitivityofdetectionofbiotin-labeledHVEMbystreptavidin-HRP.Onlyafewsimplestepsonamicrotiterplatearerequiredfortheassay.First,BTLAiscoatedona96-wellplate.Next,HVEMisincubatedwithBTLAontheplate.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionofanHRPsubstratetoproducechemiluminescence,whichcanbemeasuredusingachemiluminescencereader.UniProtQ7Z6A9Synonym(s):CD272,CD270,TNFRSF14,ATAR,HVEA,LIGHTR,TR2SuppliedAs:Thiskitcomesinaconvenient96-wellformat,withbiotin-labeledHVEM,purifiedBTLA,streptavidin-labeledHRP,andassaybufferfor100bindingreactions.Contraindications:DMSO>1%,strongacidsorbases,ionicdetergents,highsalt.Format:COMPONENTS:Instructionsforuse:SeeassaykitdatasheetfordetailedprotocolStorage/StABIlity:Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.Application(s):ThiskitisusefulforscreeninginhibitorsofHVEMbindingtoBTLAReference(s):1.DerreL.,etal.J.Clin.Invest.2010,120(1):157-167.2.Steinberg,M.W.,etal.J.Exp.Med.2008,205(6):1463-1476.Warning(s):Avoidfreeze/thawcycles.ScientificCategory:CellSurfaceReceptor/Single-passTransmembraneProteinProductType:AssayKitDatashownislot-specific.Contactusforspecificinformationonotherlots.

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