Cat#:AAR-021LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:rImmunogenPeptide(C)RPEPRGLPQ*SELNQE,correspondingtoaminoacidresidues160-174ofratα2B-adrenoceptorwithreplacementofcysteine169(C169)withserine(*S)(AccessionP19328).2ndextracellularloop.Accession(Uniprot)NumberP19328GeneID24174PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat-14/15aminoacidresiduesidentical;mouse-13/15aminoacidresiduesidentical;human-10/14aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.8mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ifc,ip,lciWesternblotWesternblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):1.Anti-α2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).2.Anti-α2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofα2B-AdrenoreceptorinratlungImmunohistochemicalstainingofratlungparaffinembeddedsectionsusingAnti-α2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021),(1:100).α2B-Adrenoreceptorisexpressedintherespiratoryepitheliumofthebronchioli(arrows).Hematoxilinisusedasthecounterstain.ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Link,R.E.etal.(1996)Science273,803.Makaritsis,K.P.etal.(1999)Hypertension33,14.Eason,M.G.andLiggett,S.B.(1992)J.Biol.Chem.267,25473.Pitcher,J.A.etal.(1998)Annu.Rev.Biochem.67,653.Kurose,H.andLefkowitz,R.J.(1994)J.Biol.Chem.269,10093.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2. Theα2B-adrenoceptorhasadistinctpatternofexpressionwithinthebrain,liverlungandkidney,andrecentstudiesusingtheknockoutmousesystemhaveshownthatdisruptionofthisreceptorindeedaffectsmouseviABIlity3,bloodpressureresponsestoα2-adrenoceptoragoNISTs3 andthehypertensiveresponsetosaltloADIng4. Liketheα2A-adrenoceptorsubtype,theα2B-adrenoceptorundergoesshorttermagonistpromoteddesensitization5.ThisdesensitizationisduetothephosphorylationofthereceptorbyG-proteincoupledreceptorkinases(GRKs)6 whichultimatelypromotesuncouplingofthereceptorfromtheG-proteinsubunit7.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:19/02/2019AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα2B-adrenoceptor.Anti-α2B-AdrenergicReceptor(extracellular)Antibody(#AAR-021)canbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2B-adrenoceptorfrommouse,rat,andhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratkidney(lanes1and5),lung(lanes2and6),liver(lanes3and7)lysatesandratskeletalmusclemembranes(lanes4and8):1.Anti-α2B-AdrenergicReceptor(extracellular) Antibody(#AAR-021),(1:200).2.Anti-α2B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

       Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。
 

       Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。