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Alomone/Anti-Adenosine A2A Receptor Antibody/AAR-002/50 µl
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Cat#:AAR-002LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,m,rImmunogenPeptide(C)RQLKQMESQPLPGER,correspondingtoaminoacidresidues201-215ofmouseadenosineA2A receptor(AccessionQ60613).3rdintracellularloop.Accession(Uniprot)NumberQ60613GeneID11540PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,human,andcanis–identical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.75mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ic,ih,wbMayalsoworkin:ifc,ipWesternblotWesternblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofAdenosine A2A Receptor in mousediagonalbandImmunohistochemicalstainingofmousediagonalbandusingAnti-Adenosine A2A ReceptorAntibody(#AAR-002).A2aR (green)appearsinthebroca ofindividualneurons(arrows)andinneuropil(asterisk). DAPIisusedasthecounterstain.ExpressionofAdenosine A2A Receptor inratlungImmunohistochemicalstainingofparaffinemeddedratlungsectionsusingAnti-Adenosine A2A Receptor Antibody(#AAR-002),(1:50).A2aRisexpressedintherespiratoryepitheliumofthebronchioli(Br).Notethatsmoothmuscleandendotheliuminbloodvesselsarenegative.Hematoxilinisusedasthecounterstain.ImmunocytochemistryRatneurospheres(Benito-Munoz,M. etal. (2016) Glia 64, 1465.).ReferencesOkusa,M.D.(2002)Am.J.Physiol.282,F10.Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.Nakata,H.(1989)J.Biol.Chem.264,16545.Cunha,R.A.(2001)NeuRochem.Int.38,107.Fredholm,B.B.etal.(2000)Naunyn-SchmiedebergsArch.Pharmacol.362,364.Canals,M.etal.(2005)J.Neurochem.92,337.Ohta,A.andSitkovsky,M.(2001)Nature414,916.Ohta,A.etal.(2006)Proc.Natl.Acad.Sci.103,13132.ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor(A1AR),AdenosineA2A Receptor(A2AAR),AdenosineA2B Receptor(A2BAR),andAdenosineA3 Receptor(A3AR).Allareintegralmembraneproteinsandaremembersofthe Gprotein-coupled receptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellular-NH2 terminus,cytoplasmic-COOHterminus,andathirdintracellularloopimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.1-3Adenosinereceptorscontrolneurotransmitterreleasethroughthe facilitatory A2AARandtheinhibitoryA1AR.4 A2AARandA1ARarethemajoradenosinereceptorsubtypesexpressedinthecentralnervoussystem(CNS).A2AARismainlyexpressedinthestriatumonGABAergicstriatopallidalneurons,whileA1ARiswidelydistributedthroughouttheCNS.5,6A2AARwassuggestedtoplayacriticalroleinattenuationofsystemicinflammatoryresponsesandpreventionofextensivetissuedamage.7 ItwassuggestedthatextracellularadenosinethataccumulatesininflamedanddamagedtissuemayactivatetheA2AARexpressedinimmunecellsleADIngtotermination/inhibitionoftheimmuneresponse.7 ItwasfurthersuggestedthatthissamemechanismmayprotecttumorsfromantitumorTcellsthroughanimmunosuppressivesignalgeneratedbytheactivationofA2AARonTcellsbyextracellularadenosineproducedfromhypoxiccanceroustissues.8Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:17/02/2019Anti-Adenosine A2A ReceptorAntibody(#AAR-002)isahighlyspecificantibodydirectedagainstanepitopeofmouseprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeA2aRfromhuman,rat,andmousesamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofrataorticendothelialcells(lanes1,3),ratbrain(lanes2,4)andJurkat(lanes5,6)lysates:1,2,5.Anti-Adenosine A2A Receptor Antibody(#AAR-002),(1:200).3,4,6.Anti-AdenosineA2A ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

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