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Alomone/Anti-Adenosine A1Receptor Antibody/AAR-006/0.2 ml
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Cat#:AAR-006LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,m,rImmunogenPeptide(C)KKVSASSGDPQKYYGKE,correspondingtoaminoacidresidues213-229ofhumanA1AR(Accession P30542).3rd intracellularloop.Accession(Uniprot)NumberP30542GeneID134PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse,rat,bovine,canisandpig-identical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.85mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ic,ih,wbMayalsoworkin:ifc,ipWesternblotWesternblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:1,2.Anti-Adenosine A1 ReceptorAntibody(#AAR-006),(1:200).3,4.Anti-AdenosineA1 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofAdenosine A1 ReceptorinratcortexImmunohistochemicalstainingofratcortexfrozensectionusingAnti-Adenosine A1 Receptor Antibody(#AAR-006).A.A1AR(green)appearsinneurons(triangles)andinastrocytes(arrows).B.Parvalbuminstaining(red)appearsincorticalinterneurons.C.ConfocalmergeofimagesdemonstratestheexistenceofA1ARinasubsetof corticalinterneuronsandastrocytes.ExpressionofAdenosine A1 ReceptorinratmedialseptumImmunohistochemicalstainingofratmedialseptumfrozensectionusingAnti-Adenosine A1 Receptor Antibody(#AAR-006).A.A1AR(green)appearsinneurons(rightpointingtriangles). B.Parvalbuminstaining(red)appearsinmedialseptalneurons.C.ConfocalmergeofdemonstratesexpressionofA1ARinasubsetofmedial septalneurons.ImmunocytochemistryExpressionofAdenosine A1 ReceptorinratDRGprimarycultureImmunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlizedratdorsalrootganglion(DRG)primaryculture.A.Stainingof DRGcellswithAnti-Adenosine A1 ReceptorAntibody(#AAR-006)(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B.Nuclearstainingofcellsusingthecell-permeabledyeHoechst33342.C.MergedimagesofAandB.F.MergedimagesofDandE.Magnification:A-C:x20D-F:x100ReferencesOkusa,M.D.(2002)Am.J.Physiol.282,F10.Nakata,H.(1989)J.Biol.Chem.264,16545.Linden,J.(1991)FASEBJ.5,2668.Park,K.S.etal.(2001)J.Pharmacol.Exp.Therap.299,501.Rice,A.M.etal.(2000)Endocrinology141,1442.LaNoue,K.F.andMartin,L.F.(1994)FASEBJ.8,72.Synowitz,M.etal.(2006)CancerRes.66,8550.Lelievre,V.etal.(1998)Eur.J.Pharmacol.341,289.Khoo,H.E.etal.(1996)CancerLett.106,17.ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionsviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor(A1AR), AdenosineA2A Receptor (A2AAR), AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopthatisimportantforbindingGproteins.1-3 Theadenosinereceptorscanbedistinguishedonthebasisoftheirdifferentialselectivityforadenosineanalogs.1-3A1ARiswidelydistributedandhasbeenwellcharacterized.HighexpressionofA1ARisfoundinthebrain(mainlyinthecortex,cerebellum,andhippocampus),dorsalhornofthespinalcord,adrenalgland,andatria,andtoalowerextentinseveralothertissuesincludingADIposetissue,thecolon,andkidney.2,4A1ARmodulatestheactivityofseveralionchannels.ActivationofA1AR(byadenosine,itsmajoragoNIST)inhibitsN-typeCa2+ channelsviaavoltage-dependent,pertussistoxin(PTX)-sensitivepathwayinneuronsoftheratmajorpelvicganglia(MPG).5SinceA1ARisthemostprominentadenosinereceptorinadipocytes,ithasbecomeanaturaltargetforresearchonobesity,whichisamajorhealthproblem.6,7 ApossIBLeroleincellproliferationandcarcinogenesishasalsobeensuggestedforA1AR.8-10Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:17/02/2019AlomoneLabsispleasedtointroduceanewantibodydirectedagainsthumanadenosineA1 receptor.Anti-Adenosine A1 ReceptorAntibody(#AAR-006)canbeusedinwesternblotanalysis,immunocytochemicalandimmunohistochemicalapplications.IthasbeendesignedtorecognizeadenosineA1Rfromrat,mouseandhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratbrain(lanes1,3)andratkidney(lanes2,4)lysates:1,2.Anti-Adenosine A1 ReceptorAntibody(#AAR-006),(1:200).3,4.Anti-AdenosineA1 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

       Alomone Labs是您离子通道研究中的伙伴,是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外:离子通道研究领域(一系列全面的离子通道研究产品线,包括标记和未标记的抗体及其相关调节因子)、G蛋白耦联受体研究领域(提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单)、 神经信号通路研究(包括一些用于神经营养因子研究的最重要的抗体和生长因子)。
 

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