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Biosensis/Rabbit antibody to MAP1LC3 B : whole serum/R-155-100/100 µl
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DescriptionMAP1LC3Bisasubunitofneuronalmicrotubule-associatedMAP1AandMAP1Bproteins,whichareinvolvedinmicrotubuleassemblyandimportantforneurogenesis.MAP1LC3Bisprobablyinvolvedinformationofautophagosomalvacuoles(autophagosomes).SUBCELLULARLOCATION:LC3-I:Cytoplasm.LC3-II:Intracytoplasmicmembrane;lipid-anchor.Cytoplasmicvesicle;autophagosome;autophagosomalmembrane;lipid-anchor.LC3-IIbindstotheautophagicmembranes.TISSUESPECIFICITY:Mostabundantinheart,brain,skeletalmuscleandtestis.Littleexpressionobservedinliver.PTM:TheprecursormoleculeiscleavedbyAPG4B/ATG4BtoformLC3-I.ThisisactivatedbyAPG7L/ATG7,transferredtoATG3andconjugatedtophospholipidtoformLC3-II.SIMILARITY:BelongstotheMAP1LC3family.BatchNo.SeeproductlabelUnitsize100µlAntigenAsyntheticpeptide(FEQRVEDVRLIC)correspondingtotheN-terminalofhumanMAP1LC3BproteinconjugatedtoBlueCarrierProteinhasbeenusedastheimmunogen.ThepeptideishomologouswiththecorrespondingsequencederivedfrommouseandratMAP1LC3Bprotein.OtherNamesMicrotubule-associatedproteins1A/1Blightchain3B;Microtubule-associatedprotein1lightchain3beta;MAP1A/MAP1BLC3B;MAP1A/1Blightchain3B;MAP1lightchain3-likeprotein2;Autophagy-relatedproteinLC3B;Autophagy-relatedubiquitin-likemodifierLC3B;APG8b;MAP-1LC3B;MAP1LC3B;MAP1ALC3;AccessionMLP3B_HUMANMLP3B_MOUSEMLP3B_RATProducedinRabbitPurityWholeserumApplicationsIHC,immunofluorescence,WB.Adilutionof1:100to1:1000dilutionisrecommendedfortheseapplications.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.SpecificityIHC,WBandELISAconfirmedthespecificityforMAP1LC3B.Cross-reactivityHuman,rat.Otherspeciesnotyettested.FormLyophilisedReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.StorageAfterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles.ExpiryDate12monthsafterpurchaseSpecificReferencesTheronAEetal.(2013)Molecularcrosstalkbetweenapoptosisandautophagyinducedbyanovel2-methoxyestrADIolanalogueincervicaladenocarcinomacells.CancerCellInt.2013Aug27;13(1):87.PilchowskiR.etal.(2011)SpecificProteinPatternsCharacterizeMetastaticPotentialofAdvancedBladderCancer.JUrol.2011Jun16.GeneralReferences1.DifilippantonioS,etal.Eur.J.Cancer39:1936-1947(2003).2.YangY.-S,etal.Endocr.Relat.Cancer10:621-627(2003).3.HeH,etal.J.Biol.Chem.278:29278-29287(2003).4.OtaT,etal.Nat.Genet.36:40-45(2004).5.TanidaI,etal.J.Biol.Chem.279:36268-36276(2004).6.TanidaI,etal.J.Biol.Chem.279:47704-47710(2004).7.TanidaI,etal.Int.J.Biochem.CellBiol.36:2503-2518(2004).

Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。

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