提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服 核对商品价格,订单生效后再付款。
Nanocs/Innovative Life Science Products and Services/100 mg/PG2-NS-35k
价格:

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
Description:N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.Physical Properties:Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;Storage Conditions:Store at -20 0C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.Reaction Procedures:Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.Materials Required:Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.Washing solution: Distilled water or any aqueous buffer.Reaction Steps:Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjugates can be purified either by size exclusion chromatography or dialysis.

PEG修饰剂(官能团)/PEG衍生物

PEG化学修饰是将聚乙二醇高分子链化学偶联到蛋白质分子上,通过增大药物分子体积以有效延长其体内半衰期,同时遮蔽其免疫位点以显著降低免疫原性。

主要用于蛋白质药物修饰,以增加体内半衰期,降低免疫原性,同时还可以增加药物的水溶性。

 

与胺基和羟基反应修饰

-NH2/ -COOH

与巯基反应修饰(-Thiol-SH

生物素修饰(Biotin

单官能团修饰(Monofunctional

双官能团修饰(Homobifunctional

表面修饰

异双官能团修饰

荧光修饰

4/8臂衍生物

纳米金

纳米金:金的微小颗粒,能与多种生物大分子结合。

纳米金标记技术:对蛋白质有吸附功能,可显微镜下观察,进行定性和半定量的免疫检测。

流式细胞仪:标记抗体

免疫印迹

免疫层析法

生物芯片:作为探针进行检测

葡聚糖/PEG包裹纳米金

生物素标记纳米金

链霉亲和素标记纳米金

BSA包裹纳米金

羟基官能团纳米金(COOH

胺基官能团纳米金(NH2

甲基官能团纳米金(CH3

叠氮化物/炔基纳米金

葡聚糖

荧光标记的葡聚糖Dextran可以作为体内细胞荧光检测材料,而像氨基修饰的葡聚糖则可以用于修饰其他的分子

荧光标记葡聚糖

罗丹明标记葡聚糖

Cy5/cy3/cy7标记葡聚糖

生物素标记葡聚糖