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HansaBioMed/ExoTEST Ready to Use Kit for Overall Exosome capture and quantification from human plasma (white plate)/HBM-

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  • ExoTESTisa sandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationinhuman plasma.

     

    Sampletype

    Plasma

    Startingmaterial

    Upto100uLofBIOLOGicalsamplecanbeloadedperwell.

    Unfractionatedplasmasamplecanbedirectlyusedforcapture.

    Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol.

    Assaytype

    Sandwichenzyme-linkedimmunoassay.

    Sensitivity

    Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein.

    Productoverview

    ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasmaandurine.

    ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit.

    Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage.

    ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies.

    Primaryantibody

    α-CD9mouseantibody

    Exosomestandard

    Lyophilizedexosomesfromplasmaofhealthydonors(see details).

    Assayprotocol

    Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection.

    Assaycalibration:Kitcontainsexosomestandardsforassaycalibration.Dependedonthesampletypeused,kitmayincludehumanplasma,serumorurineexosomes.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber.

    Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples.

    Shipmentandstorage

    Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert.

    Expirationdate

    12months.

     

    Application:

    ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.

    Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.

    SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.

    Relatedproducts :

    Exosomestandards

    RNAextractionkit

    Immunocaptureandisolationtools


    HansaBioMed抗体




    抗体是基础研究,开发人类疾病的诊断测试和疗法的重要工具。包括外泌体的细胞外囊泡(EV)表达的抗原具有3D构象和/或翻译后修饰,通常不同于细胞对应物。因此,目前市场上可买到的大多数抗体都无法以足够的敏感性和特异性识别与EV相关的抗原。HBM与Exosomics Siena Spa合作,已验证了针对常见(CD63,CD81,ALIX)和疾病特异性(癌症和神经退行性疾病)EV标记的多克隆和单克隆抗体清单


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