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QED Bioscience/Secretogranin II/26101/1 Ea
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SecretograninIIPolyclonalAntibodyORDERINGINFORMATIONCatalogNo.:26101Format:200ulrabbitantiserumBACKGROUNDSecretograninII(SgII),encodedbythegeneSCG2,isamemberofthegraninfamilyofneuroendocrinesecretoryproteins.ThegraninfamilyalsoincludeschromograninA(CgA),chromograninB(CgB),andseveralothersecretogranin-likeproteins.Studiesinrodentssuggestthatthefull-lengthprotein,SgII,isinvolvedinthepackagingorsortingofpeptidehormonesandneuropeptidesintosecretoryvesicles.SgIIoccursinavarietyofneoplasmsarisingfromendocrineandneuroendocrinecellsandcanbeausefulhistologicaltumorMarkerforthosecells.SPECIFICATIONSUMMARYAntigen:SyntheticpeptidederivedfromtheN-terminusofmatureSgIIprotein:QRNQLLGKEPDLRLENVQK(Accessionno.AAA36607).HostSpecies:RabbitSpecificity:Thisantibodyrecognizeshuman,rat,andsquirrelmonkeySgII.APPLICATIONSImmunoblotting:usediluted1:2,500-1:10,000.Abandof~86kDaisdetected.AlthoughthepredictedmwofSgIIis~71kDa,thehighlyacidicnatureofSgIIcausesittorunatahighermwinSDS-PAGE.Immunohistochemistry:usediluted1:1,000.Immunoprecipitation:usediluted1:100-1:500.Positivecontrol:ratPC12adrenalpheochromocytomacellsDILUTIONINSTRUCTIONSDiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.STORAGEANDSTABILITYThisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidrepeatedfreeze-thawcycles.PRODUCTREFERENCESThompsonMEetal.1992.ProlactingranulogenesisisassociatedwithincreasedsecretograninexpressionandaggregationintheGolgiapparatusofGHvC1cells.Endocrinology131:318-326.VasauskasAAetal.2011.RegulationanddistributionofsquirrelmonkeychorionicgonadotropinandsecretograninIIinthepituitary.GeneralandComparativeEndocrinology170:509-513.Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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