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QED Bioscience/c-Jun (Phospho-Ser79)/43026/1 Ea
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c-Jun(Phospho-Ser79)PolyclonalAntibodyORDERINGINFORMATIONCatalogNo.:43026Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.BACKGROUNDc-Junincombinationwithc-Fos,formstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunctionSPECIFICATIONSUMMARYAntigen:Peptidesequencethatincludesphosphorylationsiteofserine79(G-A-S(p)-L-K)derivedfromhumanc-JunandconjugatedtoKLH.Accessionno.:P05412,NP_002219.1HostSpecies:RabbitSpecificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatserine79.APPLICATIONImmunohistochemistry:useatdilutionof1:50-1:100.Immunofluorescence:useatdilutionof1:100-1:200.Detectionofc-Jun(phospho-Ser79)inparaffin-embeddedhumanbreastcarcinomatissue.Detectionofc-Jun(phospho-Ser79)inmethanol-fixedHeLacells.Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.DILUTIONINSTRUCTIONSDiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.STORAGEANDSTABILITYThisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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