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GoldBio/TNF-α, Human/250 μg/1130-01-250
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产品说明
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Human Tumor Necrosis FactorTNF-α, TNF-alphaCachectin, CachexinTumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active, native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.Formulation: sterile filtered white lyophilized powder. Purified and tested for use in cell culture.Sequence: MVRSSSRTPSDKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHTISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQVYFGIIALMW: ~17.5 kDa (158 amino acid)Purity: >98% by SDS-PAGEand HPLC analysesBiological Activity: Fully biologicallyactive when compared to standard. The ED50 as determined bycytotoxicity assay using murine L929 cells is less than 0.05 ng/ml in thepresence of actinomycin D.Accession Number: P01375HGNC ID: 11892Source: Escherichia coli Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions containing BSA or serum.Additional resources for this product may be found at:OMIMNOT FOR HUMAN USE!
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍
  荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子,有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光,在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。
  D-荧光素在生物技术领域是一种常用的试剂,尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠,小鼠,用来标记肿瘤细胞,干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时,非侵入性监测疾病的进程或药物效率。

 
产品具体信息
D-荧光素钾盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt
KC11H7N2O3S2
分子量:318.42 g/mol
D-荧光素钠盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
NaC11H7N2O3S2.H2O
分子量:320.32 g/mol
储存:-20℃避光保存。
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