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GoldBio/GDNF, Human/250 μg/1170-14-250
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产品说明
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!Recombinant Human Glial Cell-derived Neurotrophic FactorGDNFATF1, ATF2Astrocyte-derived trophic factorGlial cell-derived neurotrophic factor (GDNF) is a small protein that promotes the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. GDNF is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cystine knot configuration.Formulation: Sterile filtered whitelyophilized (freeze-dried) powder.Sequence: SPDKQMAVLPRRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYDKILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCIMW: ~30.1 kDa (a homodimeric protein consisting of two 134 aminoacid non-glycosylated polypeptide chains)Purity: >97% by SDS-PAGEand HPLC analysesBiological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of >5.0 × 107 IU/mg.Accession Number: P39905HGNC ID: 4232Source: Escherichia coli Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long term storage.The reconstituted sample can be apportioned into working aliquots and storedat -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutions shouldbe made in appropriate buffered solutions.Additional resources for this product may be found at:OMIMNOT FOR HUMAN USE!Note: 1000 µg volume may be delivered in multiple containers.
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍
荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子，有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光，在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。
D-荧光素在生物技术领域是一种常用的试剂，尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠，小鼠，用来标记肿瘤细胞，干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时，非侵入性监测疾病的进程或药物效率。
产品具体信息
D-荧光素钾盐
4，5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt
KC11H7N2O3S2
分子量：318.42 g/mol
D-荧光素钠盐
4，5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
NaC11H7N2O3S2.H2O
分子量：320.32 g/mol
储存：-20℃避光保存。
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