首页 > Active Motif > Active Motif/ChIP-IT® Control Kits/5 rxns/53028

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Active Motif/ChIP-IT® Control Kits/5 rxns/53028

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53028

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产品说明

ChIP is an enrichment of DNA bound by a particular protein, not a complete purification of the protein-bound DNA. As a result, ChIPs are unavoidably contaminated with chromatin bound non-specifically which can make data interpretation difficult. To solve these problems, Active Motif offers species-specific ChIP-IT® Control Kits for real time or endpoint PCR. Control kits are ideal for ChIP antibody and primer set validation when run in parallel with your test samples. The control kit will help to confirm the chromatin preparation and immunoprecipitation procedures worked properly and allow you to assess the quality of your test antibody and primer sets.The ChIP-IT® Control qPCR Kits include a positive control antibody, a bridging antibody to enhance binding affinity of mouse monoclonal antibodies, a negative control antibody to evaluate non-specific binding and species-specific positive and negative control qPCR primers. The positive and negative control primer sets enable direct comparison of gene targets for the same antibody immunoprecipitation. This eliminates the variation that is seen when comparing a ChIP antibody result to that of a negative control IgG antibody, as each have different binding affinities.Our original ChIP-IT® Control Kits are designed for use in endpoint PCR. Each kit provides positive and negative control antibodies, bridging antibody to enhance the binding affinity of mouse monoclonal antibodies, a positive control PCR primer set as well as a PCR buffer/DNA loading dye that makes your PCR reactions ready to run on the gel straight out of the thermocycler.

Active Motif 公司是一家致力于研发和生产核细胞分子生物学和表观遗传学研究用试剂的专业公司，也是世界上最大的转录因子试剂生产商和 ChIP-seq 外包服务供应商。Active Motif 的产品涉及：表观遗传学相关抗体、Chip 级别抗体、重组识别子和催化酶、预甲基化修饰的重组组蛋白、多聚核小体和生物素标记核小体；以及各类染色质分析、转录因子表达调控分析和 DNA 甲基化分析的试剂盒等。不仅如此，Active Motif 还为研究者提供专业的表观遗传学服务，特别是 Chip-seq 的外包服务。公司总部位于美国加州，目前在欧洲，日本，中国设有分公司。产品销售范围覆盖全球 30 多个国家和地区。