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Biovendor/APRIL Human Hi-5 Insect cells/RBG10066100/100 μg
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Regulatory status:RUO
Type:Recombinant
Source:Hi-5 Insect cells
Other names:TNF 13, TALL 2, TRDL 1, CD256 antigen , A Proliferation-Inducing Ligand, TNFSF13, TRDL-1α
Species:Human
Type
Recombinant
Description
APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of the colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, competes for two receptors, TACI and BCMA. APRIL has the ability to stimulate proliferation of various tumor cell lines, including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF-like receptor binding of the APRIL precursor. Recombinant human APRIL is a soluble 16.3 kDa protein, consisting of 146 amino acid residues.
Amino Acid Sequence
AVLTQKQKKQHSVLHLVPINATSKDDSDVTEVMWQPALRRGRGLQAQGYGVRIQDAGVYLLYSQVLFQDVTFTMGQVVSREGQGRQETLFRCIRSMPSHPDRAYNSCYSAGVFHLHQGDILSVIIPRARAKLNLSPHGTFLGFVKL
Source
Hi-5 Insect cells
Purity
95%
Biological Activity
Measured by its ability to induce cell death in Jurkat cells.
Storage/Expiration
–20°C
miREIA是一种基于免疫测定的新型miRNA定量方法,涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。
该测定可在常规免疫测定设备上运行,与标准临床工作流程兼容,不需要扩增步骤,并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。
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