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Biovendor/Bone Morphogenetic Protein-4 Human E. coli/RBG10085001/1 µg

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RBG10085001

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p.chemkody:after{ content:""; display:block; overflow:hidden; clear:both; height:0; } p.chemkody span:nth-of-type(7n+1) { clear: left; } p.chemkody span { float: left; min-width: 90px; margin: 0 20px 0 0; padding: 0 0 10px; color: #6e6e6e; font-family: Courier New,monospace; white-space: nowrap; }Regulatorystatus:RUOType:RecombinantSource:E.coliOthernames:BMP-4Species:HumanTypeRecombinantDescriptionBonemorphogeneticproteins(BMPs)constituteasubfamilywithintheTGF-βsuperfamilyofstructurallyrelatedsignalingproteins.Membersofthissuperfamilyarewidelydistributedthroughoutthebody,andareinvolvedindiversephysiologicalprocessesduringbothpre-andpostnatallife.LikeBMP-7,BMP-4 isinvolvedinthedevelopmentandmaintenanceofboneandcartilage.ReducedexpressionofBMP-4 isassociatedwithanumberofbonediseases,includingtheheritabledisorderFibrodysplasiaOssificansProgressiva.PeproTech’s E.coli-derivedBMP-4 isafullyactivehomodimericproteinconsistingoftwo106 aminoacidsubunits,whichcorrespondtoaminoacids303–408ofthefulllengthBMP-4 precursor.ThecalculatedmolecularweightofRecombinantHumanBMP-4(E.coli-derived)is23.9 kDa.ManufacturedusingallAnimal-Freereagents.AminoAcidSequenceKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRSourceE.coliPurity98%BIOLOGicalActivityDeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5 cells.TheexpectedED50forthiseffectis5–10 ng/ml.EndotoxinEndotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).ReconstitutionCentrifugethevialpriortoopening.Reconstitutein5mMHCl,pH3.0 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.Storage/Expiration –20°C

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。

该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。