产品说明
Alternate Name
Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic
Peptide Sequence
Ac-Tyr-Val-Ala-Asp-AFC
Appearance
Liquid
Formulation
DMSO ( 1 mM)
Molecular Formula
C₃₃H₃₆F₃N₅O₁₀
Molecular Weight
719.7
Purity
>99% by HPLC
SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C
InChi
InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)
InChi Key
GWKJISJKNKIJNP-UHFFFAOYSA-N
PubChem CID
44135090
MDL Number
MFCD01318881
Handling
Protect from light and moisture
Storage Conditions
-20°C
Shipping Conditions
Gel Pack
USAGE
For Research Use Only! Not For Use in Humans.
Description:Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.
BioVision公司是世界顶尖的细胞凋亡和信号传导产品生产商,公司总部在美丽的旧金山湾地区。公司在开发和研究细胞凋亡,代谢方面具有卓越的影响力。产品包括检测试剂盒,抗体,重组蛋白和酶等多种产品,以及其他创新的研究工具。除了细胞凋亡和代谢分析方面的传统领域,BioVision的高品质产品还覆盖细胞增殖,细胞应激,细胞损伤和修复,糖尿病,肥胖和代谢综合症,干细胞生物学,基因调控,信号转导等多个细胞生物学领域。
BioVision的众多创新性的试剂盒产品在全球享有盛誉,其Annexin V双染试剂盒至今仍然全球众多实验室被作为凋亡检测的标杆产品来使用,而种类众多的Caspase试剂盒则提供了最完整的从Caspase活性检测、Caspase蛋白定量到Caspase酶蛋白、Caspase抑制物和底物等多种不同类型的产品。
另外,BioVision的蛋白抽提和分离试剂盒也具有明显的优势和特色,包括各种蛋白提取试剂盒,如总蛋白、胞浆蛋白、胞核蛋白、线粒体、膜蛋白、细胞器蛋白提取试剂盒以及线粒体DNA提取试剂盒。BioVision还提供众多用于细胞信号转导研究的试剂盒、抗体以及拮抗蛋白等产品,以及细胞因子和生长因子等产品。BioVision提供的氧化应激和损伤类的产品也被广泛应用,其产品包括GSH, GST, NAD, NADH, NO,H₂O₂, SOD, Ascorbic, Catalase, Glutamate, Malate, Nitric Oxide等多种氧化损伤分子及指标的检测。家。
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