Alternate Name Melanoma-derived growth regulatory protein precursor, Cartilage-derived retinoic acid-sensitive protein, CD-RAP, MIA, Melanoma inhibitory activity protein Gene Symbol MIA Gene ID 8190 Accession # Q16674 Source E. coli Appearance Lyophilized protein Physical Form Description Sterile filtered and lyophilized with no additives Molecular Weight 12.2 kDa Purity by SDS-PAGE ≥98% Purity by ≥98% Endotoxin Level Biological Activity The biological activity is determined by the inhibitory effect on the invasion of Mel In Tumor cells and found active in Mel In assay. Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers Handling Centrifuge the vial prior to opening. Storage Conditions -20°C USAGE For Research Use Only! Not to be used in humans Description:MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extraCellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds.