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Rabbit anti Human FLIP (CT) (I-FLICE) (FLICE Inhibitory Protein)Catalogue number: X1128PIsotypeIgG Product Type Polyclonal AntibodyUnits100 µg HostRabbit Species reactivity HumanMouseRatApplication Western BlotWestern Blotting BackgroundApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and Ð10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIP ,,, and, respectively8.Synonyms: Casper, I-FLICE, FLAME-1, CASH, CLARPC-terminal Source Immunogen: Synthetic peptide corresponding to amino acids 447 to 464 of the C-terminal of the human FLIP /FLIPL form. Product Product Form: Unconjugated Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative Purification Method: Antigen Immunoaffiinity PurificationConcentration: See vial for concentration ApplicationsDetects FLIP by Western blot at 0.25 to 1 µg/ml.Detects a 55 kDa band in HeLa whole cell lysates.Antibody recognizes the FLIP? only.Optimal concentration should be evaluated by serial dilutions.Functional Analysis: Western BlottingPositive Control: HeLa whole cell lysates StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles Product Stability: Products are stable for one year from purchase when stored properly Shipping Conditions: Ship at ambient temperature, freeze upon arrival CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information. References1. Thome, M., et al. "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors."Nature 1997, 386, 517-5212. Irmler, M., et al. "Inhibition of death receptor signals by cellular FLIP."Nature 1997, 388, 190-195 3. Shu, H.B., et al. "Casper is a FADD- and caspase-related inducer of apoptosis."Immunity 1997, 6, 751-763 4. Hu, S., et al. "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." J. Biol. Chem. 1997, 272, 17255-17257 5. Srinivasula, S.M., et al.. "FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis." J. Biol. Chem. 1997, 272, 18542-18545 6. Goltsev, Y.V., et al. "CASH, a novel caspase homologue with death effector domains." J. Biol. Chem. 1997, 272, 19641-19644 7. Inohara, N., et al. "CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis." Proc. Natl. Acad. Sci. USA 1997, 94, 10717-10722 8. Wallach, D. "Apoptosis. Placing death under control." Nature 1997, 388, 123Protein Reference(s)Database Name: SwissProt Accession number: Q9UKL3 Species Accession: Human Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
xalpha有限公司于2000年在马萨诸塞州雪莉(美国)成立。该公司主要通过开发和生产一流的抗体来支持生命科学领域的研究人员和科学家。其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线,可提供给全球的科研实验室,制药企业,生物技术和诊断公司,医院等,目前旗下的代理公司遍布全球。
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