首页 > NSJ Bio > NSJ Bio/Anti-Bax Antibody [clone SPM336] (V9071)/7 ml/V9071IHC-7ML

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NSJ Bio/Anti-Bax Antibody [clone SPM336] (V9071)/7 ml/V9071IHC-7ML

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V9071IHC-7ML

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NSJ Bio

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DescriptionRecognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.Application NotesThe optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.ImmunogenAmino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.StorageStore the anti-Bax antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide). Citations (3) Related Products1. View all Bax antibodies

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