Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name SPM275 Purity Protein G affinity chromatography UniProt P32004 Localization Cell surface Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 1-2ug/ml Limitations This L1 antibody is available for research use only. Description:Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This mAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and schwannomas. It does not bind to pediatric or adult brain.Application Notes:The optimal dilution of the CD171 / L1 antibody for each application should be determined by the researcher.Immunogen:A homogenous suspension of 16 week human fetal brain was used as the immunogen for this CD171 / L1 antibody.Storage:Store the L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).