Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Species Reactivity Human. Others not tested. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1 Clone Name MRP1/1343 Purity Protein G affinity chromatography Buffer 1X PBS, pH 7.4 UniProt P33527 Gene ID 4363 Localization Cytoplasmic and cell surface Applications Western blot : 0.5-1ug/mlFlow Cytometry : 0.5-1ug/10^6 cellsImmunofluorescence : 1-2ug/mlImmunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Limitations This MRP1 antibody is available for research use only. Description:The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the MDR1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood.Application Notes:The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MRP1 antibody to be titered up or down for optimal performance.Immunogen:Human recombinant protein was used as the immunogen for this MRP1 antibody.Storage:Store the MRP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).