首页 > NSJ Bio > NSJ Bio/Muscle Actin Antibody (pan) [clone HHF35] (V2318)/20 ug/V2318-20UG

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NSJ Bio/Muscle Actin Antibody (pan) [clone HHF35] (V2318)/20 ug/V2318-20UG

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V2318-20UG

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DescriptionThis antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).Application NotesThe concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.ImmunogenSDS extract of human myocardium was used as the immunogen for this Muscle Actin HHF35 antibody.StorageStore the Muscle Actin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).Alternate NamesMuscle actin antibody References (2) Citations (20)Related Products1. View all Actin antibodies and conjugates

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