Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name MGND1-1 Purity Protein G affinity chromatography UniProt P15172 Localization Nuclear Applications Immunofluorescence : 1-2ug/mlImmunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)Prediluted IHC only format : incubate for 30 min at RT (2) Limitations This MyoD antibody is available for research use only. Description:MyoD/MyoD1 is a protein that plays a major role in regulating muscle differentiation. It belongs to a family of proteins known as myogenic regulatory factors (MRFs). These bHLH (basic helix loop helix) transcription factors act sequentially in myogenic differentiation. MRF family members include MyoD, Myf5, myogenin, and MRF4 (Myf6). MyoD is one of the earliest markers of myogenic commitment. It is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression is activated in response to exercise or muscle tissue damage. The effect of MyoD on satellite cells is dose-dependent; high expression represses cell renewal and induces apoptosis. Although MyoD marks myoblast commitment, muscle development is not dramatically ablated in mouse mutants lacking the gene. This is likely due to functional redundancy from Myf5. Nevertheless, the combination of MyoD and Myf5 is vital to the success of myogenesis. It is also an important effector for the fast-twitch muscle fiber (type IIa and IIx) phenotype. [Wiki]Immunogen:Recombinant human protein was used as the immunogen for this MyoD antibody.Storage:Store the MyoD antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).