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RIP3重组抗体[EPR9627]_RIP3抗体(ab209384)| Abcam中文官网
Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR9627] to RIP3 (phospho S227) Suitable for: ELISA, Dot blot, WB Reacts with: Human Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 mM z-VAD for 8 h and then harvested whole cell lysate. 常规说明 This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents. 存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. 存储溶液 pH: 7.2Preservative: 0.01% Sodium azideConstituents: 59% PBS, 40% Glycerol, 0.05% BSA The Abpromise guarantee Abpromise™承诺保证使用ab209384于以下的经测试应用 \"应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。 Receptor interacting protein 3 antibody Receptor interacting serine threonine kinase 3 antibody Receptor interacting serine/threonine protein kinase 3 antibody Receptor-interacting protein 3 antibody Receptor-interacting serine/threonine-protein kinase 3 antibody RIP 3 antibody RIP like protein kinase 3 antibody RIP-3 antibody RIP-like protein kinase 3 antibody RIPK 3 antibody RIPK3 antibody RIPK3_HUMAN antibody see all All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilutionLane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysateLane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysateLysates/proteins at 10 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionPredicted band size: 57 kDaObserved band size: 70 kDa why is the actual band size different from the predicted?Exposure time: 100 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST Dot blot analysis of RIP3 (phospho S227) phosphopeptide (Lane 1) and RIP3non-phospho peptide(Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)at 1/100,000 dilution.Blocking and diluting buffer: 5% NFDM/TBST.Exposure time: 10 seconds. Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody. All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384)Lane 1 : Untreated HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysateLane 2 : HT-29 (Human colorectal adenocarcinoma cell line) treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 uM z-VAD for 6 h and then harvested whole cell lysatePredicted band size: 57 kDaObserved band size: 70 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.Image provided by Xiaodong. Wang, National Institute of Biological Sciences, Beijing. To download a Certificate of Compliance, please enter your Lot number below:Lot number 发表研究结果有使用 ab209384?请让我们知道,以便我们可以引用本数据表中的参考文章。 ab209384 被引用在 42 文献中. Roedig J et al. USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination. EMBO Rep 22:e50163 (2021).PubMed: 33369872 Wang KJ et al. Emodin Induced Necroptosis and Inhibited Glycolysis in the Renal Cancer Cells by Enhancing ROS. Oxid Med Cell Longev 2021:8840590 (2021).PubMed: 33532038 Yin Z et al. RIPK1 is a negative mediator in Aquaporin 1-driven triple-negative breast carcinoma progression and metastasis. NPJ Breast Cancer 7:53 (2021).PubMed: 33980862 Hu H et al. RIP3-mediated necroptosis is regulated by inter-filament assembly of RIP homotypic interaction motif. Cell Death Differ 28:251-266 (2021).PubMed: 32737444 Boytard L et al. Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages. Nat Commun 11:4311 (2020).PubMed: 32855420 View all Publications for this product There are currently no Customer reviews or Questions for ab209384.Please use the links above to contact us or submit feedback about this product.Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com