共轭说明 HRP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The effect is multiple HRP molecules linked to one single antibody.
常规说明
This new and innovative HRP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional HRP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using this product. The overall staining procedure uses one less reagent (streptavidin peroxidase) and rinsing step, eliminates avidin-biotin blocking procedures and may reduce overall staining time.
The Abpromise guarantee
Abpromise™承诺保证使用ab2891于以下的经测试应用
\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
发表研究结果有使用 ab2891？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab2891 被引用在 13 文献中.
Feng C et al. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Mol Ther Nucleic Acids 20:186-195 (2020).PubMed: 32169806 Hadziselimovic F et al. Abnormal histology in testis from prepubertal boys with monorchidism. Basic Clin Androl 30:11 (2020).PubMed: 32782807 Qi Y et al. Expression of BDNF, TrkB, VEGF and CD105 is associated with pelvic lymph node metastasis and prognosis in IB2-stage squamous cell carcinoma. Exp Ther Med 18:4221-4230 (2019).PubMed: 31777532 Zhang W et al. CaSR participates in the regulation of vascular tension in the mesentery of hypertensive rats via the PLC-IP3/AC-V/cAMP/RAS pathway. Mol Med Rep 20:4433-4448 (2019).PubMed: 31485595 Mesci P et al. Blocking Zika virus vertical transmission. Sci Rep 8:1218 (2018).PubMed: 29352135 View all Publications for this productThank you very much for contacting us with your question.
Ab2891 is a prediluted antibody, and it is intended to be used without further dilution, on both frozen and paraffin sections. We don\'t have larger quantities at this time, but if you need 60 mL or more (10+ bottles), we can arrange a bulk order for you. Please contact sales@abcam.com if this is the case.
I hope this information will be useful, but please let me know if you have further questions or need anything else and I\'ll be happy to help.
Thank you for contacting us.
The expiry date would be 1 year i.e. untill 31st July 2013.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Thank you for contacting us.
Ihave have received another replyfrom the supplying lab, with a few more details for question 2) especially:
#1) Detection polymer composition is proprietary and that detail cannot be disclosed.
#2) We have not run our polymer detection side-by-side with a non-polymer HRP detection to allow us to give a quantitative answer. We can say that the polymer detection affords a significant increase in sensitivity that will allow users to increase primary antibody titers and/or decrease incubation times.
I hope this information is of some more help.Please do not hesitate to contact us if you need any more advice or information.
Thank you for contacting us.
I have heard back from the supplying lab:
Unfortunately, the answer to the first question (number of HRP molecules per polymer) is proprietary.
As for the secondquestion (% increase in signal intensity compared to regular HRP-secondary antibody): The answer would be subject tomany varying conditions, such asfixation, signal in tissue, section of tissue, HIER, etc. We do not have a general answer that applies to these circumstances in this case.
I am very sorry that I do not have more detailed information which I can give you. Please do not hesitate to contact us if you need any more advice or information.
Unfortunately, we are not sure if this product contains the Fc fragment.
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Unfortunately since it has not been previously tested in Western blot and since we do not know the concentration of the secondary antibody (this information is considered confidential by the antibody originator), I can only make a guess at the dilution you would need to use. You may wish to try a few different dilution, I might try a range from 1/10 to 1/100 to begin with, and then see what the staining looks like and adjust the dilutions accordingly.Please do let us know how you make out with this antibody in Western blot, as we would love to know whether it will work in this application and what dilution can be used.I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
This antibody is a polymer detection for mouse and rabbit antibodies on human tissue. It will cross-react with virtually all monoclonals and rabbits antibodies. Specific cross-reactivity with a particular protein has not been observed. As I mentioned on the telephone, the concentration, protein carrier and preservative are proprietary.I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
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Further to correspondence with the source of this antibody unfortunately I have been unable to determine a precise concentration. They are unable to disclose the antibody concentrations of their detection systems. I can however tell you that this product is provided in a pre-diluted, ready-to-use format. I am sorry that I cannot be of more assistance. Read More
I am terribly sorry for the delay in finding the answer to your question regarding the concentration of ab2891.Unfortunately the originator of this antibody cannot disclose this information to us as it is proprietary and must remain confidential.I apologise for the inconvenience, Read More
This product is provided in a pre-diluted, ready-to-use format for immunostaining procedures. There is no need to dilute further this secondary antibody. Please refer to our IHC protocols for more information, and if you have any further questions, please do let us know. Read More
Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
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