提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服
核对商品价格，订单生效后再付款。
Cytoskeleton/MICAL-Oxidized (Pyrene labeled) Actin Protein (>95% pure): Rabbit Skeletal Muscle/2 x 250 ug/MPAX1
价格：
自营商城
解放采购
正品保障
及时交付
厂家直采
一站服务
货号：
品牌：
会员服务：
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考，请联系客服询价。
免费咨询热线
4000-520-616
产品说明
DetailsMICAL interacts with F-actin and uses NADPH as a cofactor to oxidize actin at Met44 and Met47 (b-actin nomenclature). Functionally, oxidation of Met44 has a profound effect on actin polymerization because the residue resides in the D-loop of subdomain 2 of the protein, which is critical for actin subunit contacts; thus, upon oxidation, Met44 becomes negatively charged and interferes with actin monomer-monomer interaction and promotes F-actin severing and depolymerization. Regulation of actin oxidation at Met44/Met47 has been shown to destabilize F-actin in vivo and to play a key role in a growing number of cellular processes. As part of the MOXtrue™ product line, Pyrene labeled rabbit skeletal muscle actin protein (MICAL-oxidized) (MXAP95) has been enzymatically oxidized at methionines 44 and 47 with the MICAL flavoprotein monoxygenase protein. Purified MICAL-oxidized (pyrene labeled) actin has reduced susceptibility to subtilisin A cleavage at M47/G48 by > 90%, and has also been validated for downstream applications such as actin polymerization assays. To learn more about using MICAL-oxidized Actin as a research tool see our datasheet To learn more about the MICAL/MsrB/Actin physiological redox system see our Newsletter Each lot of purified protein is quality controlled to provide high batch to batch consistency, see COA documents. Validated Applications Pyrene labeled Actin Protein (MICAL-Oxidized) Purity DeterminationA 50 μg sample of pyrene labeled actin protein (MICAL-oxidized) was separated by electrophoresis in a 4- 20% tris-glycine gel and stained with Coomassie Blue. Protein quantitation was performed using the Precision RedTM Protein Assay Reagent (Cat. # ADV02). Mark12 standard molecular weight markers are from Invitrogen.Subtilisin Assay on MICAL-Oxidized Pyrene Actin vs Native Pyrene ActinPyrene labeled actin (Cat. # AP05) and MICAL-oxidized (pyrene labeled) actin (Cat. # MXAP95) was diluted to 0.1 mg/ml (2.3 μM). 2 μg of each sample was then left untreated, or treated with subtilisin (1:200 w/w) for 15 min. Samples were then separated by SDS-PAGE and visualized with Coomassie staining. Click here for a detailed method Actin Polymerization of Oxidized Pyrene Actin Versus Native Pyrene ActinPyrene-labeled actin (Cat # AP05) and MICAL-oxidized (pyrene labeled) actin (Cat. # MXAP95) were diluted to 0.1 mg/ml (2.3 μM) or 0.2 mg/ml (4.6 μM) (see method). Samples were then incubated with 2x polymerization buffer. Upon actin polymerization fluorescence was detected with a spectrophotometer. A.U. = arbitrary unitsClick here for a detailed method For more information contact: signalseeker@cytoskeleton.comAssociated Products:MOXtrue™ 6xHis MICAL-1 Protein (Cat. # MIC01)MOXtrue™ 6xHis MsrB2 Protein (Cat. # MB201)MOXtrue™ MICAL-oxidized Actin (Cat. # MXA95)Rabbit Skeletal Muscle Actin (Cat. # AKL95)Pyrene Labeled Rabbit Skeletal Muscle Actin (Cat.# AP05)AboutFor product Datasheets and MSDSs please click on the PDF links below.Certificate of Analysis: Lot 001CitationsFor the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click hereFaqsVisit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。
Cytoskeleton，Inc.很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于纯化蛋白的生产和易于使用的试剂盒，以研究生化和细胞过程。我们的试剂盒既可用于基础研究或小型筛查的少量样品，也可用于大型筛查的高通量规模
除了我们现有的产品外，我们还提供产品系列中微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白的药物筛选服务。有关更多信息，请参见我们的药物筛选服务页面。
如果您想从市场上购买到特定产品，请随时与我们联系。我们在这里为您提供帮助。
由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。
新品排行榜
1
Cytoskeleton/Cdc42 Pull-down Act...
2
Cytoskeleton/Arf6 Pull-down Acti...
3
Cytoskeleton/Actin polymerizatio...
4
Cytoskeleton/Signal-Seeker™ Acet...
5
Cytoskeleton/Laminin (Green fluo...
6
Cytoskeleton/Arf1 G-LISA Activat...
7
Cytoskeleton/Arf6 G-LISA Activat...
8
Cytoskeleton/Cdc42 G-LISA Activ...
9
Cytoskeleton/MICAL-Oxidized Acti...
10
Cytoskeleton/His-Arf6 Protein: a...
文章排行榜
1
The function and clinical application of extracellular...
2
Nitric oxide and cGMP signal transduction positively...
3
JWA Antibody (PA5-18094)
4
DNA-templated synthesis of biomimetic cell wall for nano...
5
Rho Family and Rap GTPase Activation Assays
6
Dynamic Mechano-Regulation of Myoblast Cells on...
7
Dynamic Mechano-Regulation of Myoblast Cells on...
8
beta Catenin Antibody (PA1-37255)
9
DNA-templated synthesis of biomimetic cell wall for nano...
10
线粒体荧光探针大全:TMRM,Mitotracker,JC-1 - 其他 - 资讯 - 蚂蚁淘
微信公众号
手机扫码，关注动态