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Cytoskeleton/6xHis MsrB2 Protein aa: 42-182 (2 x 50 micrograms)/1 x 1 mg/MB201-XL
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4000-520-616
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TheMsrfamilyarewellconservedproteinsthatfunctiontoreduceoxidizedmethioninetoL-methionine.TheSelR(MsrB)proteinwasidentifiedastheenzymeresponsIBLeforreductionoftheMICAL-oxidizedMet44andMet47aminoacidsofactin..AspartoftheMOXtrue™productline,humanMSRB2protein(MB201)[accession#Q9Y3D2(MSRB2_HUMAN)]hasbeenproducedandpurifiedfromabacterialexpressionsystem.TherecombinantproteinisN-terminally6xHistidinetaggedandiscomprisedofaminoacids42-182.ValidationstudieshaveshownthatMB201canreduceMICAL-oxidizedactinwhichwillenhancesubtilisinAcleavageby>90%inlimitedproteolysisassays.MB201hasalsobeenvalidatedfortheproductionofreducedactinwhichcanbeusedindownstreamapplicationssuchassedimentationassaysandactinpolymerizationassays.TolearnmoreaboutusingMsrB2asaresearchtoolseeourdatasheetTolearnmoreabouttheMICAL/MsrB/ActinphysiologicalredoxsystemseeourNewsletterEachlotofpurifiedproteinisqualitycontrolledtoprovidehighbatchtobatchconsistency,seeCOAdocuments.ValidatedApplicationsMsrB2ProteinPurityDeterminationA10μgsampleofMsrB2proteinwasseparatedbyelectrophoresisina4-20%tris-glycinegelandstainedwithCoomassieBlue.ProteinquantitationwasperformedusingthePrecisionRedTMProteinAssayReagent(Cat.#ADV02).Mark12standardmolecularweightMarkersarefromInvitrogen.SubtilisinAssayonMsrB2TreatedOxidizedActinMICAL-oxidizedactin(MetOActin)(Cat#MXA95)wasdilutedto0.1mg/ml(2.3mM)andleftuntreated,ortreatedwithDTT(10mM),MB201(0.3mM),oracombinationofboth(seemethod).2mgofeachsamplewasthenleftuntreated,ortreatedwithsubtilisin(1:200w/w)for15min.SampleswerethenseparatedbySDS-PAGEandvisualizedwithCoomassiestaining.ClickhereforadetailedmethodOxidizedActinSedimentation+/-MsrB2TreatmentMICAL-oxidizedactin(MetOActin)(Cat.#MXA95)wasdilutedto0.2mg/ml(4.6mM)or0.8mg/ml(18.4mM)(seemethod).Sampleswerethenincubatedwith2xpolymerizationbufferor2xpolymerizationbuffersupplementedwithMB201+DTTfor1hatroomtemperature.Sampleswerespuninanultracentrifugeat100,000gfor1.5h.SampleswerethenseparatedbySDS-PAGEandvisualizedwithCoomassiestaining.ClickhereforadetailedmethodOxidizedActinPolymerization+/-MsrB2TreatmentPyrene-labeledactin(Cat#AP05)andMICAL-oxidizedpyrenelabeledactin(Cat.#MXAP95)weredilutedto0.1mg/ml(2.3mM)(seemethod).Sampleswerethenincubatedwith2xpolymerizationbuffersupplementedwithnothing(labeledControl),DTTalone(labeled+DTT),MB2010.3µM+DTT(labeled+MB2010.3),orMB2011.2µM+DTT(labeled+MB2011.2),.Uponactinpolymerizationfluorescencewasdetectedwithaspectrophotometer(seemethodbelow).A.U.=arbitraryunitsClickhereforadetailedmethodFormoreinformationcontact:signalseeker@Cytoskeleton.comAssociatedProducts:MOXtrue™6xHisMICAL-1Protein(Cat.#MIC01)MOXtrue™MICAL-oxidizedActin(Cat.#MXA95)MOXtrue™MICAL-oxidizedPyreneLabeledActin(Cat.#MXAP95)RabbitSkeletalMuscleActin(Cat.#AKL95)PyreneLabeledRabbitSkeletalMuscleActin(Cat.#AP05)
Cytoskeleton，Inc.成立于1993年。自成立以来，我们一直在不断扩大产品范围。
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由于我们的科学家在各自的专业领域都有多年的工作经验，因此我们能够提供高质量的产品。自1993年成立以来，我们以合理的价格生产优质的产品而闻名。
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